高效液相色谱法测定芪芨散中黄芩苷的含量

目的：建立芪芨散中黄芩苷的高效液相色谱测定方法。方法采用Agilent C18（150 mm ×4.6 mm,5μm）柱分离,甲醇-水-三乙胺（50∶50∶0.25）（磷酸调节pH值3.0）进行洗脱,在277 nm处测定芪芨散中黄芩苷的含量。结果黄芩苷在11.28~90.24μg/ml范围内质量浓度与峰面积呈良好的线性关系,平均回收率为99.38%（相对标准偏差=0.78%,n=9）。结论本方法准确度高,简便,重现性好,可作为芪芨散的质量控制方法之一。

High Performance Liquid Chromatography in Determination of Baicalin Content in Qiji San Medication

Objective To establish high performance liquid chromatography （HPLC） in detection of Baicalin content in Qiji San. Methods The content was detected in Agilent C18（150 mm × 4. 6 mm, 5 μm） column disassoci-ation and elution with methanol-water-triethylamine （50∶ 50∶ 0. 25, adjusted to pH 3. 0 phosphoric acid at the 277 nm of detection wavelength. Results The good linear correlation between mass concentration and peak area was obtained in the concentration range of 11. 28-90. 24 μg/ml, and the average recovery was 99. 38% （ relative standard deviation=0. 78%, n=9）. Conclusion The method is accurate and simple with good reproducibility, and can be used to control the quality of Qiji San medication.