HPLC法测定鞣酸蛋白原料及制剂中鞣酸的含量

目的：建立HPLC法测定鞣酸蛋白原料及制剂中鞣酸的含量。方法：使用Techmate C18-ST（4.6 mm ×250 mm，5μm）色谱柱；流动相A为0.5％冰醋酸溶液，流动相B为乙腈，流量0.8 mL· min^-1，梯度洗脱；柱温30.0℃；检测波长272 nm；进样量10μL；使用SPSS19.0软件设计正交试验，最优前处理条件为加入6 mol· L^-1盐酸溶液10 mL，5％乙醇5 mL，105℃水解6 h。结果：线性范围为0.001～0.3 mg· mL^-1（r＝0.9999），平均加样回收率为97.0％，RSD＝0.5％（n＝9）。结论：本方法简便、准确、专属性强，可用于测定鞣酸蛋白原料及制剂中鞣酸的含量。

Determination of Tannic Acid in Albumin Tannate and Its Preparations by HPLC

Objective：To establish an HPLC method for the determination of tannic acid in albumin tannate and its preparations.Methods：The column was Techmate C18-ST （4.6 mm ×250 mm, 5μm）.The mobile phase A was 0.5%acetic acid and the mobile phase B was acetonitrile ,the flow rate was 0.8 mL· min^-1 with gradient elution;The column temperature was 30.0℃and the injection volumn was 10μL with a detection wavelength at 272 nm.Orthogonal test was designed by SPSS 19.0 software and results showed that the optimal pretreatment conditions was to join 10 ml of 6 mol· L^-1 hydrochloric acid solution and 5 ml of 5%ethanol, hydrolyzing for 6 hours at 105℃.Results： The linear range was 0.001-0.3 mg · mL^-1 （ r=0.9999 ） , the average recovery rate was 97.0% and RSD=0.5（n=9）.Conclusion： The method is simple, accurate and suitable for determining the content of tannic acid in albumin tannate and its preparations.