共表达基因疫苗MAGE-1/IL-18的抗肿瘤免疫治疗作用

目的观察已构建的共表达基因疫苗pcIL-18-MAGE诱导特异性免疫应答的能力和抗肿瘤免疫治疗作用。方法共表达疫苗肌注小鼠，间隔十天加强免疫一次，共免疫三次，以pcMAGE-1、 pcIL-18、pcDNA3和PBS为对照，检测小鼠脾细胞上清液IL-12和IFN-γ的浓度、脾细胞CTL杀伤活性及小鼠T细胞亚群情况；观察基因疫苗免疫MAGE (+)小鼠的成瘤时间和生存时间。结果pcIL-18-MAGE质粒免疫的小鼠，其脾细胞对SMMC-7721的杀伤率最高，与各对照组相比，差异有统计学意义；脾细胞CD3⁺、CD4⁺、CD8⁺ T细胞和上清液中细胞因子IL-12和IFN-γ均明显升高。共表达疫苗免疫MAGE (+)小鼠后，小鼠成瘤时间明显延迟，生存时间明显延长。结论共表达基因疫苗pcIL-18-MAGE在实验动物体内有显著的诱导特异性抗肿瘤免疫作用，且对肿瘤的生成有一定的抑制作用。

Anti-tumor Immunotherapy Effect of Co-expression Genetic Vaccine MAGE-1/IL-18

Objective To observe specific immune response and anti-tumor immunotherapy effect of the co-expression genetic vaccine MAGE/IL-18. Methods Co-expression genetic vaccine was injected to mice,two booster injections were carried out at ten days intervals. Using pcMAGE-1, pcIL-18, pcDNA3 and PBS as comparison, IL-12, IFN-γ, CTL killing activity and T cell subgroup were investigated. Tumor growth and survival rates of vaccine immunized MAGE(+) mice were recorded. Results The mice which were immunized by pcIL-18-MAGE, compared with control groups, had the highest CTL killing activity, CD3~+,CD4~+,CD8~+ T cells,IL-12 and IFN-γ all step up obviously. The co-expression vaccine can delay tumor growth time and prolong the live time for mice. Conclusion Co-expression genetic vaccine pcIL-18-MAGE can induce conspicuous and has specific anti-tumor immunotherapy effect on laboratory animals, and can significantly reduce the tumor growth.