反义硫代寡核苷酸对人脑胶质瘤细胞系转化生长因子α基因表达 …

目的 研究转化生长因子α反义寡核苷酸对人脑胶质细胞系中核ＴＧＦα基因表达的作用。

Antisense oligodeoxynucleotide of TGF alpha inhibits its gene expression and proliferation of a human glioma cell line

OBJECTIVE: To study the effect of antisense oligodeoxynucleotide(ON) of TGF alpha on its gene expression in TJ 905 cell line. METHODS: Antisense and sense TGF alpha phosphorothioate ON (SON) and random SON were synthesized and transfected to TJ 905 cells mediated by lipofectin. Their effects on the TGF alpha gene expression were examined by in situ hybridization of TGF alpha mRNA, immunohistochemical study and cell count. RESULTS: (1) TGF alpha antisense SON could significantly inhibit the growth of TJ 905 cell line. The inhibition peaked at 24 hour after transfection, the inhibition rate reached 68% at 20 microM, and the effect decreased 72 hours after transfection. The inhibition effect was dose dependent. (2) Antisense TGF alpha SON inhibited TGF alpha expressions at both mRNA and protein levels. CONCLUSION: Antisense TGF alpha SON can inhibit the expressions of TGF alpha and markedly inhibit cell growth of human glioma TJ 905 cell line. TGF alpha contributes in the growth potential of glioma.