低频超声联合尿激酶体外溶栓效果观察

目的:观察低频超声与尿激酶联合应用对体外血栓的促溶作用,并对其作用机理进行探讨。方法:制备全血血栓块按照不同实验方法分组:(1)生理盐水组(NS组);(2)尿激酶组(UK组);(3)超声组(US组);(4)超声联合尿激酶组(UK+US组)进行处理,检测处理后的血块重量丢失比和纤维蛋白降解产物D-D二聚体含量评价各组处理的溶栓效果。结果:US组与NS组相比血块重量丢失比和D-D二聚体含量的差异均有统计学差异意义,说明低频超声有明确的促溶作用;UK+US组的血块重量丢失比和纤维蛋白降解产物D-D二聚体含量相比UK组或US组均有显著的统计学差异。结论:低频超声可促进尿激酶的溶栓作用。

The Thrombolytic Effects of Urokinase in Combination with Low-Frequency Ultrasound in Vitro

Objective:To observe thrombolytic effects of urokinase in combination with low-frequency ultrasound in vitro and discuss its mechanism.Methods:To prepare whole blood clots and according to the treatmentand divide the clots into 4 groups:(1) normal saline group(NS group);(2) urokinase group(UK group);(3) ultrasound group(US group);(4) ultrasonic combined with urokinase group(UK+US group).After treatment clot lysis was evaluated by each group's clot mass loss and the concentrations of D-dimer a fibrin degradation product.Results:Comparing with the NS group,the US group has significant statistical differences in clot mass loss and the concentrations of D-dimer,it shows that low-frequency ultrasonic can enhance the clot lysis.Comparing with UK or US group alone,the group of ultrasonic combined with urokinase group showed significant statistical differencesclot mass loss and the concentrations of D-dime.Conclusion:Low-frequency ultrasound coould accelerate thrombolysis effects of urokinase in vitro.