肾小球内皮细胞培养及产生细胞外基质的实验研究

作者成功地培养了肥儿及牛肾小球内皮细胞，将内皮细胞克隆后传代培养了７代，并利用间接免疫荧光方法进行鉴定，观察胎儿及牛肾小球内皮细胞都可以表达第ＶＩＩＩ因子相关抗原，纤维连接蛋白，ＩＶ型胶原及层粘连蛋白。测定了不同时胎儿肾小球内皮细胞培养上清中细胞外基质的水平。结果证实不加任何刺激因子，肾小球内皮细胞可以稳定地产生细胞外基质。

Experimental studies of glomerular endothelial cellculture and its Production of extracellular matrixes

e successfully cultured human fetal and bovineglomerular endothelial cells by cell cloning and main-tained seven passages. The glomerular endothellal cellswere identified by indirect immunofluorescence method.We found that the fetal and bovine glomerular endothe-lial cells could produce factor VIII related antigen, fi-bronectin, type IV collagen and laminin. The extracel-lular matrixes in the cultured supernatant were deter-mined at various stages. The results showed that theglomerular endothelial cells could produce extracellularmatrixes steadily without any stimulating factor.