| 溶性苯二氮受体的制备与分析 |
| |
| 引用本文： | 林军,胡本荣.溶性苯二氮受体的制备与分析[J].中山大学学报(医学科学版),1989(2). |
| |
| 作者姓名： | 林军 胡本荣 |
| |
| 作者单位： | 中山医科大学药理学教研室
(林军)，中山医科大学药理学教研室(胡本荣) |
| |
| 摘 要： | 采用新型去垢剂CHAPS制备兔大脑前叶皮质溶性苯二氮(艹卓)受体。溶膜受体放射配基结合分析,见苯二氮(艹卓)受体具有可饱和性,与3~H]FNP结合的亲和力KD_1为0.31nml/L,KD_2为6.7nmol/L。光亲和标记结合SDS电泳测得亚基分子量为55000;凝胶过滤层析测得受体分子量为632000。
|
| 关 键 词： | 苯二氮受体 溶脱 去垢剂 分子量 |
Characterization of Soluble Benzodiazepine Receptors |
| |
| Lin Jun Hu Benrong.Characterization of Soluble Benzodiazepine Receptors[J].Journal of Sun Yatsen University(Medical Sciences),1989(2). |
| |
| Authors: | Lin Jun Hu Benrong |
| |
| Institution: | Department of Pharmacology |
| |
| Abstract: | The benzodiazepine receptors in frontal lobes of cerebral cortex of rabbits were solu-bilized with the detergent 3- (3eholamidopropyl)-dimethylammonio propaneslfonate (CHAPS). 3H flunitrazepam binding to soluble receptors was saturable and showed nonlinear Scatchard plot with KD1 0.31 nmol/L and KD2 6.7 nmol/L. The molecular weight of the soluble benzodiazepine receptors determined by SDS-PAGE after photoaffinity labelling was 55 000, by chromatography on Sephadex G 200 was 623 000. |
| |
| Keywords: | Benzodiazeipne receptors Solubilization CHBPS Molecular weight |
| 本文献已被 CNKI 等数据库收录！ |
