RP-HPLC法同时测定舒肝祛脂胶囊中4种大黄蒽醌的含量

目的建立同时测定舒肝祛脂胶袭中4种大黄蒽醌类成分大黄酸、大黄素、大黄酚和大黄素甲醚含量的高效液相色谱方法。方法采用反相高效液相色谱法，Diamonsil C18色谱柱（5μm，4．6mm×150mm），流动相：甲醇-0．1％磷酸溶液（77：23），检测波长：254nm，柱温：30℃，流速：1．0mL／min，进样量20μL。结果缌陆范围分别为：大黄酸0．0832～2．08gg／mL、大黄素0．1008-2．52gg／mL、大黄酚0．2912—7、28μg／mL和大黄素甲醚0．088～2．20μg／mL。平均加样回收率分别为：大黄酸97．3％、大黄素96．9％、大黄酚96．5％和大黄素甲醚95．9％。结论本方法灵敏，重现性好，适合于舒肝祛脂胶袭中这4种蒽醌含量的分析。

Simultaneous Determination of 4 Anthraquinones in Shugan Quzhi Capsules by RP-HPLC

Objective To establish a RP-HPLC method for simultaneous quantitative determination of 4 anthraquinones in Shugan Quzhi capsules. Methods A RP-HPLC method was used with a Diamonsil C18 column （5 μm, 4.6 mm× 150 mm）. The mobile phase was composed of methanol and 0.1% H3PO4 water solution （77 ： 23, v/v）. The wavelength of UV detector was 254 nm and column temperature was 30 ℃. Flow rate was 1.0 mL/min and 20 μL was injected every time. Results The linear ranges of rhein, emodin, chrysophanol and physcion were 0.083 2-2.08, 0.100 8-2.52, 0.291 2-7.28 and 0.088-2.20 μg/mL, respectively. Their average recoveries were 97.3%, 96.9%, 96.5% and 95.9%, respectively. Conclusion This method was sensitive, repeatable and suitable to determine the contents of 4 anthraqulnones in Shugan Quzhi capsules.