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| 乙肝病毒adr型基因疫苗诱导小鼠体液免疫应答 | |
| 引用本文： | 景洪标,余宏宇,戴益民,姚玉成. 乙肝病毒adr型基因疫苗诱导小鼠体液免疫应答[J]. 第二军医大学学报, 2001, 22(9): 891-893 |
| 作者姓名： | 景洪标 余宏宇 戴益民 姚玉成 |
| 作者单位： | 1. 第二军医大学长海医院病理科病理学教研室 2. 长征医院病理科 3. 基础医学部细胞生物学教研室 |
| 基金项目： | 国家自然科学基金资助项目 ( 396 70 6 6 9) |
| 摘 要： | 目的：构建乙肝病毒adr型基因疫苗pCMVS2-S，观察其免疫小鼠的特异性体液免疫应答。方法：将构建的乙肝病毒基因疫苗注射于C57BL/6小鼠胫骨前肌肉，运用ELISA检测不同时间小鼠血清中抗HBs抗体。结果：注射基因疫苗1周后，血清中抗HBs抗体转阳，1个月后抗体达到高峰，并以高水平维持至少2个月。结论：基因疫苗pCMVS2-S诱导C57BL/6小鼠产生较好的体液免疫应答。 |
| 关 键 词： | 乙型肝炎病毒 adr型基因疫苗 体液免疫应答 小鼠 |
| 文章编号： | 0258-879(2001)09-0891-03 |
| 修稿时间： | 2001-02-25 |
Humoral immune response to gene vaccine against adr hepatitis B virus |
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| JING Hong-Biao,YU Hong-Yu,DAI Yi-Min,YAO Yu-Cheng. Humoral immune response to gene vaccine against adr hepatitis B virus[J]. Former Academic Journal of Second Military Medical University, 2001, 22(9): 891-893 | |
| Authors: | JING Hong-Biao YU Hong-Yu DAI Yi-Min YAO Yu-Cheng |
| Affiliation: | JING Hong-Biao1,YU Hong-Yu2,DAI Yi-Min1,YAO Yu-Cheng3 |
| Abstract: | Objective: To construct HBV adr gene vaccine pCMVS 2-S and to observe the specific humoral immune responses in C57BL/6 mice after gene immunization. Methods: The HBV gene vaccine was injected into tibialis anterior muscles of C57BL/6 mice. ELISA was used to detected the anti-HBs antibody in mice sera at various time points after gene transfer. Results: Anti-HBs in sera of mice was tested positive 1 week after plasmid injected, the level of antibody peaked 4 weeks later, and kept high titer for at least 2 months. Conclusion: The good response of humoral immunity can be induced by injection of pCMVS 2-S in C57BL/6 mice. |
| Keywords: | hepatitis B virus gene vaccine humoral immune response |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |