流式细胞免疫表型测定在诊断骨髓增生异常综合征中的意义

应用骨髓和外周血的形态学及细胞遗传学检查可对有明确病态造血和异常核型的骨髓增生异常综合征（MDS）患者做出诊断。然而，仍有相当数量的患者缺乏上述证据。最近的一些研究表明，可以应用流式细胞仪（FCM）检测MDS患者骨髓造血细胞表面免疫标记以反映其系特异性和阶段特异性紊乱，从而将MDS与其他非恶性血细胞减少性疾病区分开。本文对这一领域的相关研究进展，诸如FCM在MDS中的应用、FCM参数与MDS形态学及细胞遗传学诊断的相关性及FCM参数与MDS分型和预后的关系等进行了综述。

Role of Flow Cytometric Immunophenotyping in Diagnosis of Myelodysplastic Syndromes-Review

Bone marrow morphology and karyotyping are effective ways to diagnose myelodysplasitic syndromes.However,there are still many patients without these two abnormalities.Recently,there are considerable amount of evidence showing that flow cytometric immunophenotyping is a good approach to detect the abnormal differentiation and maturation of different cell lines and maturation stages of bone marrow cells in MDS patients.The highly reproducible results allow to distinguish MDS from other non-clonal hematocytopenia disorders and have a diagnostic advantage especially in case of low-risk MDS.Meanwhile,it showed a good correlation with the morphology and karyotyping,and with the International Prognostic Scoring System(IPSS) playing a prognostic role.This article reviewed recent advances of reseach in this field such as the application of FCM in MDS detection,the correlation of FCM parameters with morphologic and cytogenetic diagnosis of MDS as well as the relationship of parameters with typing and prognosis of MDS and so on.