高剂量碘对小鼠免疫功能的影响

目的:观察给予不同剂量碘对小鼠特异性免疫和非特异性免疫功能的变化,探讨高剂量碘对免疫功能的影响。方法:用160只BaLB/c雌性小鼠,按体重随机分4组,每组40只,在自来水中加入不同剂量碘酸钾,使其含碘1500、3000、6000μg/L,以自来水为对照,自由饮水,30d后测定小鼠脏体比,淋巴细胞增殖反应能力,T细胞表面主要辅助分子CD4+、CD8+比值的变化,CD4+T细胞Th1,Th2比值的变化,半数溶血值,抗体生成细胞数,NK细胞活性,腹腔巨噬细胞吞噬鸡红细胞的吞噬率、吞噬指数。结果:含碘6000μg/L组的脾脏指数、半数溶血值、抗体生成细胞数,CD4+/CD8+与正常对照组相比降低,淋巴细胞增殖反应能力、IFN-γ/IL-4、NK细胞活性与正常对照组相比升高,差异显著。腹腔巨噬细胞吞噬鸡红细胞的吞噬率、吞噬指数及胸腺指数在各剂量组间的差异无统计学意义。结论:碘与机体免疫反应相关,小鼠饮水中含碘达到6000g/L时即可造成免疫功能紊乱,机体存在自体免疫性疾病发生的危险。

EFFECT OF EXCESS POTASSIUM IODATE ON IMMUNE FUNCTION IN MICE

Objective: To Investigate the effect of iodine on specific immunity and nonspecific immunity in mice, and the relationship between excess dosage iodine and immune system. Method: 160 female BaLB/c mice were divided into 4 groups randomly according to their body weight, 40 for each group ,10 of each group was considered as one batch for test . Mice in 4 groups were orally administrated with tap water (control) and 1 500,3 000,6 000 g/L iodine respectively for 30 d. They were used to observe spleen index , thymus index, lymphocyte proliferation response,CD4 /CD8 ,Th1/Th2,50% haemolysis value, antibody formation cell, phagocyte function, the activity of natural-killer cells(NK). Results: In iodine 6 000 g/L group, spleen index ,thymus index, 50% haemolysis value, CD4 /CD8 were significantly lower, and lymphocyte proliferation response, Th1/Th2,the activity of NK cell were significantly higher than those in control group. There were no significant differences about phagocyte function, thymus index among all dosages. Conclusion: Iodine as an exogenous chemical material is related to immune reaction, and the dosage 6 000 g/L can induce disorders of immune function in mice. It may have a central role in the development of autoimmune disease.