间质性膀胱炎大鼠动物模型膀胱黏膜保护前后组胺受体变化及意义

目的 探讨透明质酸膀胱灌注治疗间质性膀胱炎(IC)后膀胱组织中组胺受体变化的意义. 方法 IC模型大鼠20只,随机分为2组,每组10只.实验组尿道灌注0.8 g/L透明质酸.对照组即时处死.HE染色计数膀胱固有层单核炎症细胞,特殊染色计数肥大细胞.免疫组化染色2组大鼠膀胱组织,IPP4.5图像分析软件计算吸光度(A)值,比较2组间的差异. 结果 实验组单核炎症细胞数和肥大细胞数分别为(12.20±2.48)、(2.90±0.87)个/视野;组胺H1～H4受体A值分别为0.015±0.007、0.006±0.001、0.007±0.004、0.061±0.026;对照组单核炎症细胞数和肥大细胞数分别为(23.90±3.07)、(7.08±1.23)个/视野;组胺H1～H4受体A值分别为0.055±0.033、0.031±0.023、0.033±0.017、0.091±0.059.2组单核炎症细胞和肥大细胞数比较差异均有统计学意义(P＜0.01);H1、H2、H3受体A值明显减小(P＜0.05);组胺H4受体A值差异无统计学意义(P＞0.05). 结论 组胺H1、H2、H3受体参与IC发生,其受体拮抗剂可能用于临床治疗IC.

Variation and significance of histamine receptors before and after the protection to the mucous membrane of urinary bladder of interstitial cystitis rat model

Objective To investigate the changes of histamine receptors in bladder before and after the treatment by sodium hyaluronate in rats of interstial cystitis (IC). Methods Twenty IC model rats were randomly divided into experimental group and control group, with 10 for each group.The bladders in experimental group were filled with sodium hyaluronate, while the rats in control group were executed at once. The bladders were dyed with HE staining, special staining and immunohistochemistry staining to count the number of mononuclear cells and mast cells and observe the changes of histamine receptors. Results In experimental group,the counts of mononuclear cells and mast cells were 12.20±2.48 and 2.90±0.87 respectively;the numbers of average optical of histamine receptor H1, H2, H3, H4 were 0.015±0.007, 0.006±0.001, 0.007±0.004, 0.061±0.026 , respectively. In control group, the counts of mononuclear cells and mast cells were 23.90±3.07 and 7.08±1.23;the numbers of average optical of histamine receptor H1, H2, H3, H4 were 0.055±0.033, 0.031±0.023, 0.033±0.017, 0.091±0.059, respectively. The differences of mononuclear cells and mast cells were significant between the 2 groups(P＜0.01). The differences of average optical of histamine receptor H1, H2, H3 between the 2 group were significant (P＜0.05), while the difference of histamine receptor H4 was not significant(P＞0.05). Conclusion Histamine receptor H1, H2, H3 take part in the development of IC, the antagons may be used for the treament of IC.