珊瑚复合人骨髓基质细胞构建组织工程骨

目的：观察人骨髓基质细胞在角孔珊瑚上立体培养后的生长情况及其在体内的成骨能力。方法：穿刺抽吸人髂骨区骨髓基质细胞，体外培养扩增、诱导后，接种于角孔珊瑚中立体培养5d，观察细胞在角孔珊瑚表面的贴附及伸展情况；将上述细胞/角孔珊瑚复合物植入体内，观察植入物在体内的成骨情况。结果：细胞可在角孔珊瑚中立体培养成活。体内植入后4周，复合物中有少量新骨形成；体内植入后8周，复合物中出现大量较成熟骨组织；而对照组4、8周均无骨组织形成。结论：穿刺抽吸的人骨髓基质细胞可作为骨组织工程的种子细胞，角孔珊瑚可作为骨组织工程的支架材料。

Tissue-Engineering Bone Fabricated By Seeding Human Bone Marrow Stromal Cells On Porous Coral

Objective: To investigate the ability of the growth of human bone marrow stromal cell (HBMSC) on coral in vitro and new bone formation of HBMSC/coral composite in vivo. Method: Human bone marrow collected from adult ilium by needle aspiration was cultured in vitro to expand and differentiate the stromal cells. Scanning electron microscopy was used to evaluate the growth of HBMSC on coral in vitro. The HBMSC/coral composites were implanted subcutantously into athymic nude mice (coral alone as control). At 4 and 8 weeks after implantation, the effectiveness of bone formation was evaluated by means of gross, histological observation. Result: HBMSC could adhere and spread well on the surface of coral. 4 weeks after implantation, some new bone was observed in the composite. 8 weeks after implantation, the new-formed bone became mature and invasion of bone marrow was observed in some area. In coral alone group, all specimens showed no new bone formation. Conclusion: HBMSC can be used as seeded cells and coral can be used as a kind of scaffold in bone tissue engineering.