| PCR检测鼠疫菌种中F1抗原基因 |
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| 引用本文： | 赵斌,王忠惠,邹艳波,邴琪. PCR检测鼠疫菌种中F1抗原基因[J]. 中国地方病防治杂志, 2010, 0(3): 177-178 |
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| 作者姓名： | 赵斌 王忠惠 邹艳波 邴琪 |
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| 作者单位： | 吉林省地方病第一防治研究所; |
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| 摘 要： | 目的应用PCR检测试验用EV菌株是否存在caf1基因。方法应用含内部对照PCR技术,以鼠疫菌特异基因caf1合成一对引物,进行PCR实验。结果该试验用EV菌株经PCR扩增,呈现一条与caf1基因分子量相吻合的清晰目的扩增带。结论该试验用EV菌株未缺失产生F1抗原的基因,可以用于提取F1抗原等试验。
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| 关 键 词： | 耶尔森氏鼠疫菌 PCR F1抗原 65MD质粒 caf1基因 |
To detect F1 antigen gene by PCR in the Yersinia pestis strains |
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| ZHAO Bin,WANG Zhong-hui,ZOU Yan-bo,et al. To detect F1 antigen gene by PCR in the Yersinia pestis strains[J]. Chinese Journal of Control of Endemic Disenaces, 2010, 0(3): 177-178 |
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| Authors: | ZHAO Bin WANG Zhong-hui ZOU Yan-bo et al |
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| Affiliation: | ZHAO Bin,WANG Zhong-hui,ZOU Yan-bo,et al(The First Institute of Endemic Disease of Jilin Province,Baicheng 137000) |
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| Abstract: | Objective To detect caf1 gene by PCR in the experimental EV strains.Methods According to caf1 gene ,we have designed a couple of primer and dectected by PCR with internal control.Results The EV strains have been amplified by PCR,there were a same band with anticipative caf1 gene.Conclusions The experimental EV strains have not lose F1 antigen gene,it can be used for F1 antigen extraction and other experiments. |
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| Keywords: | The Yersinia pestis PCR F1 antigen 65MD plasmid caf1 gene |
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