肾移植术后受者人巨细胞病毒感染与其它机会感染的研究

应用聚合酶链反应（ＰＣＲ）技术检测６５例肾移植受者（ＲＴＲ）尿中人巨细胞病毒（ＨＣＭＶ）－ＤＮＡ，配合捕获ＥＬＩＳＡ法及间接ＥＬＩＳＡ法检测ＨＣＭＶ－ＩｇＭ及ＩｇＧ，诊断ＨＣＭＶ感染，感染率为６０％（３９／６５）。应用临床及实验室方法诊断其它病原体（如革兰氏细菌，结核杆菌，霉菌，ＨＳＶ及ＶＺＶ等）所致的机会感染，结果表明，ＨＣＭＶ感染组机会感染率明显高于ＨＣＭＶ未感染组（Ｐ＜０．０１）。对其机理的分析表明，ＨＣＭＶ感染后外周血Ｔ细胞亚群产生了变化，即ＨＣＭＶ感染组ＣＤ４水平显著低于ＨＣＭＶ未感染组，ＣＤ８水平显著增高，导致ＣＤ４／ＣＤ８值显著降低（Ｐ均小于０．０１），说明ＨＣＭＶ感染组患者细胞免疫受到严重抑制，招致其它病原体的机会感染。

The Effects of Human Cytomegalovirus Infection on Circulating T Cell Subsets and Clinical Sequelae in Renal Transplant Recipients

Out of 65 cases of renal transplant recipents 39 cases (60%) were diasnosed as humam cytomegalovirus (HCMV)by polymerase chain (PCR)and enzyme linked immunosorbent assay (ELISA).Circulating T cell subsets were also as sayed.The result indicated that HCMV infection seriously affacted circulating T cell subsets,and resulted in CD4/CD8 ratio decreased or inversed(HCMV infectious group:l.3810.019;noinfectious group:l.98f 0.045, P<0.of).Theimmunosuppressive effects of HCMV infection on resipients had led to serious clinical seqelae ,the frequence of oppoortunistic infection (gram -bacteria ,trberculosis ,fugus ,herpes simplex viruses was higher in HCMV infectious group than noinfectious group(P<001)