无呼吸机支持下小鼠主动脉弓缩窄模型优化法评价

目的 研究在无呼吸机支持下小鼠主动脉弓缩窄模型优化法,比较其与传统模型构建方法在手术存活率方面的差别.方法 取雄性C57BL/6小鼠90只进行TAC模型建立,分为假手术组30只,无呼吸机手术组30只,呼吸机手术组30只,比较3组术后1周总存活率.结果 假手术组无1只死亡；无呼吸机手术组术中死亡4只,术后1周死亡0只,总存活率86.7％；呼吸机手术组术中死亡9只术后1周内死亡2只,总存活率63.3％.无呼吸机手术组和呼吸机手术组比较差异有统计学意义（P =0.037 ＜0.05）.两手术组术后4周的心脏超声检测均获得理想的心肌肥厚模型.结论 优化后的无呼吸机支持下小鼠主动脉弓缩窄模型的手术方法较传统方法手术存活率更高,手术方式更为便捷.

An Optimized Method of Transverse Aortic Constriction in Mice without Ventilator

Abstract : Objective To compare an optimized method of transverse aortic constriction (TAC) without ventilator and the traditional TAC by the survival rate of mice in one week. Methods Ninety C57/BL6 mice were randomly divided into sham-operated group (n=30), TAC without ventilator group (n=30) and TAC with ventilator group (n=30). The survival rates were recorded in 1 week and the cardiac function was evaluated by using ultrasonic instrument 4 weeks after the operation. Results The mice survival rate was higher in the TAC group without ventilator than in that with ventilator, and the new improved method resulted in the same cardiac hypertrophic model after 4 weeks. Conclusions The new optimized method of TAC without ventilator is better for improving survival rate and more convenient than the traditional TAC.