| 乙型肝炎病毒在慢性乙型肝炎患者肝细胞染色体上的整合情况 |
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| 引用本文： | 夏海鸣,吴引伟,殷国庆.乙型肝炎病毒在慢性乙型肝炎患者肝细胞染色体上的整合情况[J].江苏医药,2006,32(3):202-203. |
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| 作者姓名： | 夏海鸣 吴引伟 殷国庆 |
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| 作者单位： | 210003,南京,东南大学医学院附属南京市第二医院;210003,南京,东南大学医学院附属南京市第二医院;210003,南京,东南大学医学院附属南京市第二医院 |
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| 基金项目： | 江苏省南京市医学重点专科基金 |
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| 摘 要： | 目的研究慢性乙型肝炎患者肝细胞染色体是否存在HBV的整合及整合情况。方法选择我院慢性乙型肝炎患者16例，采用荧光原位杂交（FISH）技术制备肝细胞染色体标本，以地高辛标记的HBV探针进行原位杂交。结果16例患者中，5例（31．25％）肝细胞分裂期染色体上可观察到绿色荧光信号，HBV呈低频率整合（1．00％～1．50％），整合位点呈随机分布。结论应用FISH技术可检测肝细胞染色体上整合型HBV。慢性乙型肝炎患者肝细胞染色体上存在整合型HBV，使HBV感染可能发展成为一种遗传性传染性疾病，并成为肝癌高发的重要危险因素。
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| 关 键 词： | 荧原位杂交 慢性乙型肝炎 乙型肝炎病毒 病毒整合 |
| 收稿时间： | 2005-03-08 |
| 修稿时间： | 2005-03-08 |
HBV virus integration in hepatocellular chromosome of patients with chronic hepatitis B |
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| XIA Haiming,WU Yinwei,YIN Guoqing.HBV virus integration in hepatocellular chromosome of patients with chronic hepatitis B[J].Jiangsu Medical Journal,2006,32(3):202-203. |
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| Authors: | XIA Haiming WU Yinwei YIN Guoqing |
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| Institution: | Affiliated Nanjing Second Hospital, Medical College of Southeast University, Nanjing 210003, CHINA |
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| Abstract: | Objective To study the integration of HBV on hepatocellular chromosome of the patients with chronic hepatitis B.Methods Chromosome fluoresent in situ hybridization(FISH) was done with dig-labeled HBV probe in 16 patients with chronic hepatitis B.Results Green fluorescent signals were seen in 5 patients(31.25%),the integration sites were present in low frequency(1.00%-1.50%) and randomly distributied.Conclusion FISH technique could be used for detecting the HBV integration on the hepatocellular chromosome.HBV integrating into hepatocellular chromosome of the patients with chronic hepatitis B not only can lead to a result of hereditary diseases,but also is a high risk factor for liver cancer. |
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| Keywords: | Fluorescence in situ hybridization Chronic hepatitis B Hepatitis B virus Virus integration |
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