新西兰兔糖尿病肾病模型的建立方法

目的: 探讨小剂量四氧嘧啶(ALX)建立新西兰兔糖尿病肾病(diabetic nephropathy,DN)模型的可行性。方法: 雄性新西兰兔20只,8只为对照组,给予常规饲料,12只为糖尿病组,给予高糖高脂饮食(常规饲料加5%蔗糖、5%猪油和1%胆固醇)喂养2周,再加用小剂量ALX耳缘静脉注射,分别检测造模前与造模12周后兔血糖、血脂、尿蛋白和肾功能指标,并于12周后进行组织形态学观察。结果: 建模成功动物具有"多饮、多食、多尿、体重减轻"的特点,并出现DN相应的形态及功能改变。结论: 高脂高糖饮食加ALX药物诱导的方法,12 周时可成功制备新西兰兔2型DN模型。

Construction of a New Zealand rabbit model of diabetic nephropathy

AIM: To establish a method to produce animal model of diabetic nephropathy(DN) in New Zealand rabbits using alloxan(ALX).METHODS: Twenty male New Zealand rabbits were randomly divided into 2 groups: 8 rabbits in control group were fed with conventional feed such as buffer solution;12 in diabetes milleuts(DM) group were fed with high-sugar and high-fat diet(conventional feed plus 5% sucrose,5% pork fat and 1% cholesterol) for 2 weeks,then intravenous injection of ALX at 50 mg/kg was given,and another dosage of 100 mg/kg ALX was intravenously injected after 48 h.The levels of blood glucose,blood cholesterol,triglyceride,urine microalbumin,and serum creatinine were detected before and 12 weeks after the processes of modeling.After 12 weeks,the rabbits were sacrificed,and the kidneys were taken for pathological examination.RESULTS: The successful model of DN in the rabbits had the characteristics of high blood glucose,abundant urine microalbumin(P<0.01),and corresponding morphological and functional changes of the kidneys.CONCLUSION: Rabbits with high-sugar and high-fat diet plus twice intravenous injection of ALX at a total dose of 150 mg/kg(50 mg/kg and 100 mg/kg,respectively) shows low mortality and stable diabetes mellitus state.This method induces a typical DN model in 12 weeks.