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| 应用蛋白质组学和组织芯片研究annexin A2在胃癌中的表达 | |
| 引用本文： | 李秀娟,刘桂桃,张志强,温浩,张桂英,李茂玉.应用蛋白质组学和组织芯片研究annexin A2在胃癌中的表达[J].中国病理生理杂志,2012,28(4):619-624. |
| 作者姓名： | 李秀娟 刘桂桃 张志强 温浩 张桂英 李茂玉 |
| 作者单位： | 1. 新疆医科大学第一附属医院消化科, 新疆 乌鲁木齐 830011; 2. 新疆医科大学基础医学院病理生理学教研室, 新疆 乌鲁木齐 830011; 3. 新疆医科大学第五附属医院职业病科, 新疆 乌鲁木齐 830011; 4. 中南大学湘雅医院消化科, 湖南 长沙 410008; 5. 中南大学湘雅医院卫生部蛋白质组学重点实验室, 湖南 长沙 410008 |
| 基金项目： | 国家自然科学基金资助项目,新疆维吾尔自治区自然科学基金资助项目,新疆维吾尔自治区高校科研计划青年教师科研启动基金资助项目,自治区卫生厅青年科技人才专项科研项目 |
| 摘 要： | 目的: 研究胃癌中膜联蛋白A2(ANXA2)的差异表达及其与胃癌临床病理参数的关系。方法: 激光捕获显微切割技术(LCM)纯化15例胃腺癌细胞(GAC)和其癌旁正常胃黏膜上皮细胞(NGEC),采用18O/16O分别标记2种细胞样本酶切后的多肽混合物。纳升级反相液相色谱串联质谱(Nano-RPLC-MS/MS)定量鉴定GAC和NGEC的差异表达蛋白质。Western blotting验证差异蛋白ANXA2的表达。免疫组化方法检测组织芯片中75对胃癌组织和癌旁组织中ANXA2的表达,分析ANXA2表达与胃癌临床病理参数的关系。结果: 共筛选出78个差异表达蛋白质,其中ANXA2蛋白的表达水平在GAC中较NGEC中明显上调(2.32∶ 1),Western blotting检测ANXA2蛋白表达在GAC组升高(P<0.01);免疫组化显示:ANXA2的表达与胃癌的浸润深度、淋巴结转移、分化程度、TNM分期和肿瘤大小相关(P<0.01),与年龄、性别和远处转移无关(P>0.05)。结论: ANXA2蛋白的上调可能在胃癌的生物学行为中发挥重要作用。 |
| 关 键 词： | 胃肿瘤 膜联蛋白A2 蛋白质组 |
| 收稿时间： | 2011-11-20 |
Study of annexin A2 expression in gastric cancer by proteomics and tissue microarray |
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| LI Xiu-juan , LIU Gui-tao , ZHANG Zhi-qiang , WEN Hao , ZHANG Gui-ying , LI Mao-yu.Study of annexin A2 expression in gastric cancer by proteomics and tissue microarray[J].Chinese Journal of Pathophysiology,2012,28(4):619-624. | |
| Authors: | LI Xiu-juan LIU Gui-tao ZHANG Zhi-qiang WEN Hao ZHANG Gui-ying LI Mao-yu |
| Institution: | 1Department of Gastroenterology,The First Affiliated Hospital,2Department of Pathophysiology,College of Preclinical Medicine,3The Occupational Diseases Prevention and Treatment Institution,The Fifth Affiliated Hospital,Xinjiang Medical University,Urumqi 830011,China;4Department of Gastroenterology,5Key Laboratory of Cancer Proteomics of Ministry of Health of China,Xiangya Hospital,Central South University,Changsha 410008,China) |
| Abstract: | AIM: To investigate the differential expression of annexin A2(ANXA2) in gastric carcinoma and to analyze the relationship between ANXA2 expression and clinicopathological parameters of gastric carcinoma.METHODS: Pure gastric adenocarcinoma cells(GAC) and normal gastric epithelial cells(NGEC) in 15 patients with gastric cancer were acquired by laser capture microdissection(LCM).All peptide specimens after trypsin digestion were labeled with 18O/16O.Quantitatively identification of differential expression of the proteins betweem GAC and NGEC was performed by Nano-RPLC-MS/MS.The expression of ANXA2 in the 2 kinds of tissues was detected by Western blotting.Tissue microarray containing 75 pairs of gastric carcinoma and para-carcinoma tissues was used and the expression of ANXA2 in these specimens was detected by the method of immunohistochemistry(IHC).The relationship between ANXA2 expression and clinicopathological parameters of the pateints with gastric carcinoma was analyzed.RESULTS: A total of 78 differential proteins were identified and ANXA2 was up-expressed in GAC(2.32∶1),which was confirmed by Western blotting(P<0.01).The results of IHC showed that the correlations between the expression level of ANXA2 protein and invasive depth(T stage),lymph node metastasis(N stage),histological differentiation,TNM stage and the size of tumor were observed(P<0.01),but the correlations between the ANXA2 expression and sex,age and distant metastasis(M stage) were not found(P>0.05).CONCLUSION: The up-expressed ANXA2 may play an important role in the biological behavior of gastric cancer |
| Keywords: | Stomach neoplasms Annexin A2 protein Proteome |
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