骨形成蛋白4基因与NOG基因的交互作用与非综合征性唇腭裂的关系

目的:探讨骨形成蛋白4基因538T/C(BMP4 538T/C)变异与NOG基因615G/C(NOG 615G/C)变异之间交互作用在非综合征性唇腭裂(nsCL/P)发生中的意义。方法:从两家综合性医院中选择200名nsCL/P患者和200名对照,两组在年龄、性别、家庭社会经济状况方面相匹配。应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测BMP4538T/C、NOG 615G/C的多态性;运用相加模型分析BMP4 538T/C与NOG 615G/C变异之间的交互作用。结果:用非条件Logis-tic回归模型调整父母亲怀孕时的年龄和文化程度后,BMP4 538T/C与NOG 615G/C变异之间对nsCL/P患病具有正交互作用,归因交互效应2.05,归因交互效应百分比46.28%,纯因子间归因交互效应百分比59.77%,交互效应指数2.49。结论:BMP4538T/C与NOG 615G/C变异之间交互作用在该研究人群nsCL/P患病中具有重要意义。

Interaction between bone morphogenetic protein 4 gene and NOG gene and the relationship with non-syndromic cleft lip and palate

Objective:To explore the significance of interaction between bone morphogenetic protein 4(BMP4) 538T/C gene mutation and NOG 615G/C gene mutation in the occurrence of non-syndromic cleft lip and palate(nsCL/P).Methods:A total of 200 patients with nsCL/P and 200 normal persons(control group) were selected from two general hospitals.The patients in the two groups were matched in terms of age,gender,familial factor,social factor,and economic factor.PCR-RFLP was used to detect the polymorphisms of BMP4 538T/C and NOG 615G/C;additive model was applied to analyze the interaction of BMP4 538T/C mutation and NOG 615G/C mutation.Results:After adjusting ages and educational levels of parents when they got pregnancy by unconditional logistic regression model,there was positive interaction between BMP4 538T/C mutation and NOG 615G/C mutation in the patients with nsCL/P.The attributable interaction effect was 2.05,the percent of attributable interaction effect was 46.28%,the percent of attributable interaction effect between pure factors was 59.77%,and the interaction index was 2.49.Conclusion:The interaction between BMP4 538T/C mutation and NOG 615G/C mutation has important significance for researching the population with nsCL/P.