| HPLC法测定盐酸羟嗪含量及有关物质 |
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| 引用本文： | 刘海燕,崔艳,严拯宇.HPLC法测定盐酸羟嗪含量及有关物质[J].海峡药学,2009,21(12):59-61. |
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| 作者姓名： | 刘海燕 崔艳 严拯宇 |
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| 作者单位： | 中国药科大学分析化学教研室,南京,211198 |
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| 摘 要： | 目的建立高效液相色谱法测定盐酸羟嗪含量及有关物质的方法。方法采用C18柱,以磷酸氢二铵（0.01mol.L-1,pH6.0）-乙腈（60：40）为流动相,流速为1mL.min^-1,检测波长为230nm,进样量20μL,柱温30℃。结果在浓度为5～50μg.mL^-1范围内线性关系良好（r=0.9995,n=6）,检测限为0.0083μg.mL^-1。结论本法简便、准确、灵敏度高、重现性好,可作为盐酸羟嗪含量及有关物质的测定方法。
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| 关 键 词： | 盐酸羟嗪 含量测定 有关物质 HPLC法 |
Determination of content and related substances of Hydroxyzine Hydrochloride by HPLC |
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| LIU Hai-yan,CUI Yan,YAN Zheng-yu.Determination of content and related substances of Hydroxyzine Hydrochloride by HPLC[J].Strait Pharmaceutical Journal,2009,21(12):59-61. |
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| Authors: | LIU Hai-yan CUI Yan YAN Zheng-yu |
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| Institution: | (China Pharmaceutical university , Nanjing, 211198, China) |
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| Abstract: | OBJECTIVE To establish a HPLC method for the determination of the content and related sub- stances of Hydroxyzine Hydroehloride. METHODS The HPLC system is consisted of C18 column, the mobie phase is Ammonium hydrogen phosphate（0.01tool· L^- 1, pH6.0） ： acetonitrile（60 ： 40）. the flow rate was 1.0mL· min^- 1. Detection wavelength was set at 230nm, the injection volume was 20μL and column temperature was 30℃ RESULTS The calibration curve was linear in the range of 5.01 -- 50.10μg·mL^- l r = 0. 9995 （n = 6） ; the limit of detection was 0. 0.083μg·mL^- 1. CONCLUSION The method was rapid, simple, accurate sensitive and suitable for the content and related substances determination of Hydroxyzine Hydrochloride. |
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| Keywords: | Hydroxyzine Hydrochloride Content Related substances HPLC |
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