血浆IL-18和Hcy及hsCRP与2型糖尿病大血管并发症的相关性研究

目的研究血浆白细胞介素18（IL-18）、同型半胱氨酸（Hcy）、超敏C反应蛋白（hsCRP）与2型糖尿病大血管并发症的关系。方法收集本院住院的2型糖尿病患者（50例）血浆标本为T2DM组,以健康志愿者血浆标本（20例）作为正常对照组。糖尿病患者又分为大血管并发症组（30例）及非大血管并发症组（20例）。采用ELISA法测定IL-18,高效液相色谱法测定Hcy。IL-18与Hcy、hsCRP的相关性用简单相关分析,用多元逐步线性回归分析血浆IL-18的影响因素。结果T2DM组较正常对照组血浆IL-18、Hcy、hsCRP明显升高;大血管病变组血浆IL-18、Hcy、hsCRP与非大血管病变组、正常对照组血浆IL-18、Hcy、hsCRP比较,差异有统计学意义（P〈0.05）;IL-18与Hcy、年龄、hsCRP、FPG、2hPG呈正相关;T2DM组为整体,IL-18为因变量,Hcy、hsCRP等14个因素为自变量,进行多元逐步线性回归分析,最后Hcy、FPG、hsCRP进入回归方程。结论IL-18、Hcy、hsCRP与2型糖尿病大血管并发症有关,IL-18和Hcy、hsCRP具有相关性。

The relationship among interleukin-18, homocysteine ,high-sensitivity C-reactive protein and macrovascular complication in type 2 diabetes

Objective To study the relationship among interleukin-18,homocysteine,high-sensitivity C-reactive protein and macrovascular complication in type 2 diabetes.Methods Seventy specimens of plasma were collected from 50 patients with type 2 diabetes,and 20 matched healthy subjects in Tongji hospital,from June to December 2005.Fifty cases of type 2 diabetes were divided into macrovascular complications subgroup(30 cases) and non macrovascular complications subgroup(20 cases).Plasma concentrations of Interleukin-18 were measured in EDTA-anti-coagulanted plasma using ELISA,and Hcy was detected with high-performance liquid chromatography.The relationship among interleukin-18,homocysteine,high-sensitivity C-reactive protein were analyzed by Bivariate Correlations analysis.The factors related to interleukin-18 in type 2 diabetes were analyzed by multiple stepwise Linear Regression analysis.Results Plasma IL-18,Hcy,hsCRP concentrations were significantly higher in type 2 diabetic patients than that in age-matched control subjects.Plasma IL-18,Hcy,hsCRP concentrations were significantly higher in macrovascular complications subgroup,compared with non macrovascular complications subgroup and health control subjects.Multiple stepwise Linear Regression analysis showed that plasma Hcy,FPG and hsCRP were independently factors and significantly correlated with Plasma IL-18.Conclusion Interleukin-18,Hcy and hsCRP might be important risk factors for macrovascular complications in type 2 diabetes,and plasma IL-18 positively correlated with plasma Hcy and hsCRP.