| 布鲁氏菌和O:9型小肠结肠炎耶氏菌两种抗体鉴别的实验研究 |
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| 引用本文： | 鲁齐发,武素怀,王晓英,郭宝岚,张伟,李兰玉,马烨,何利华.布鲁氏菌和O:9型小肠结肠炎耶氏菌两种抗体鉴别的实验研究[J].中华流行病学杂志,1993,14(5):283-286. |
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| 作者姓名： | 鲁齐发 武素怀 王晓英 郭宝岚 张伟 李兰玉 马烨 何利华 |
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| 作者单位： | 中国预防医学科学院流行病学微生物学研究所 102206 北京市 |
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| 基金项目： | 本项研究为国家自然科学基金资助项目 |
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| 摘 要： | 采用ＳＡＴ、ＥＬＩＳＡ及ＤＩＢＡ分别对６种布鲁氏菌及耶氏菌Ｏ：９型感染的兔血清抗体的检查结果表明，无论用布鲁氏菌１０４Ｍ、耶氏菌Ｏ：９型菌集抗原做ＳＡＴ检查或用布鲁氏菌１６Ｍ超声波破碎抗原做ＥＬＩＳＡ检查均对布鲁氏菌与耶氏菌Ｏ：９型的兔血清抗体无鉴别意义；而用１６Ｍ可溶性蛋白抗原、外膜蛋白抗原以及用耶氏菌Ｏ：３型可溶性蛋白抗原、外膜蛋白抗原做ＥＬＩＳＡ或ＤＩＢＡ均能对布鲁氏菌与耶氏菌Ｏ：９型的兔血
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| 关 键 词： | 布鲁氏杆菌 结肠炎耶氏菌 抗原 |
| 收稿时间： | 1993/1/30 0:00:00 |
A Preliminary Study on Serological Differentiation between Antibody against Brucella and that against Yersinia enterocolitica Serotype O:9 |
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| Lu Qifa.A Preliminary Study on Serological Differentiation between Antibody against Brucella and that against Yersinia enterocolitica Serotype O:9[J].Chinese Journal of Epidemiology,1993,14(5):283-286. |
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| Authors: | Lu Qifa |
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| Institution: | Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing 102206 |
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| Abstract: | Standard agglutination test (SAT), enzyme-linked immunosorbent assay (ELISA), dot immunobinding assay (DIBA) have been developed for the detection of antibodies against 6 strains of Brucella and that against Yersinia enterocolitica Serotype O:9. The results showed that differentiation could be made between Brucella and Yersinia O:9 when 16M SPA, OMP and Y O:3 SPA. OMP were used by ELISA, but not when 104M and YO:9 antigens were used by SAT. So, 16M and YO:3 OMP antigens were better for detection of antibodies against Brucella and Yersinia O:9 by ELISA. |
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| Keywords: | Brucella Yersinia enterocolitica ELISA Antigen Antibody |
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