宣威及昆明地区肺癌患者cyclinD1基因多态性比较及意义

目的探讨eyclinD1（CCND1）基因在肺癌发生、发展中的作用。方法采用PCR-SSCP和DNA直接测序技术，对宣威、昆明地区非小细胞肺癌各45例（分别为宣威、昆明组）和非肺癌（肺大疱、肺出血）行手术切除的正常肺组织石蜡包埋标本18例（对照组）进行CCNDI的G870A基因多态性检测。结果CCND1的G870A的从、AG、GG基因型在宣威组的频率分别为46．7％、42．2％、11．1％；在昆明组中分别为24．4％,44．4％、31．1％；对照组为22．2％、38．9％、38．9％。CCND1的G870A的从型发生频率宣威组与对照组比较有显著差异（P〈0．05），而昆明组与对照组比较无差异。CNND1的G870A基因多态性与TNM分期及淋巴结转移有关（P〈0．05）。结论肺癌的发生、发展是一个多基因、多步骤参与的过程，CCND1的G870A多态性与宣威地区特异性有关。

Comparative study of polymorphism of cyclinD1 gene in patients with non-small cell lung cancer from Xuanwei and Kunming

Objective To study the role of cyelinD1 （CCND1） gene in occurrence and development of lung cancer. Methods 108 cases were collected, which included 45 patients from Xuanwei with NSCLC ,45 patients from Kunming with NSCLC and 18 patients with pneumatocele and pneumorrhagia（ control group）. PCR-SSCP and DNA sequence were used to analyze CCND1 polymorphism of extra 4/intra 4 （G870A） in the samples of paraffin-embedded human lung tissues from these 108 residing patients. Results AA, AG, GG of G870A genetype probability in Xuanwei was 46.7%, 42.2%, 11.1% ; which in Kunming was 24.4% ,44.4% ,31.1%, in control group was 22.2% ,38.9% ,38.9%, respectively. AA of G870A genetype total probability in Xuanwei was higher than that in Kunming. Conclusions The occurrence and development of lung cancer is a mulfi-gene, multi-step process of development, the G870A polymorphism of CCND1 was related to the zero sepeeifity.