高住低练对大鼠肝组织低氧诱导因子-1α蛋白表达的影响

目的:探讨不同持续时间低氧后运动训练(高住低练)对大鼠肝组织低氧诱导因子-1α(HIF-1α)蛋白表达的影响。方法:将60只雄性SD大鼠随机分为6组,每组10只:安静对照组不训练,不进行低氧暴露;两低氧暴露组和两高住低练组每天分别进行8小时或12小时低氧暴露(氧浓度12.6%,相当于海拔4000m);同时,训练对照组和两高住低练组每天均以25m/min的速度进行跑台训练1h,每周5天,共4周。采用免疫组织化学的方法检测各组大鼠肝组织HIF-1α的蛋白表达,并采用计算机显微图像分析系统进行HIF-1α定位及阳性表达定量分析。结果:与安静对照组相比,不论是单纯低氧暴露组、单纯训练组或者高住低练组HIF-1α蛋白表达均显著增加(P<0.05);12小时低氧暴露组HIF-1α蛋白表达灰度值比8小时稍增高,无统计学意义(P>0.05),而阳性物质表达面积和PI显著增加(P<0.05);与训练对照组比较,8小时和12小时高住低练组HIF-1α蛋白表达增加,具有统计学意义(P<0.05,P<0.01);8小时高住低练组HIF-1α蛋白表达比8小时低氧暴露组显著增加(P<0.05),12小时高住低练组HIF-1α蛋白表达比12小时低氧暴露组显著增加(P<0.05,P<0.01);与8小时高住低练组相比,12小时高住低练组HIF-1α蛋白表达显著增加(P<0.05)。结论:(1)不论单纯低氧暴露、单纯训练或者高住低练均能促进肝组织HIF-1α蛋白表达增加;(2)高住低练过程中肝组织HIF-1α蛋白表达高于单纯低氧暴露或单纯训练方式,不同持续时间低氧后运动对大鼠肝组织HIF-1α蛋白表达的影响不同。

Effects of HiLo Training on the Hypoxia Induced Factor-1α Expression in Rats Liver

The effects of HiLo(living at high altitude /training at low altitude) on the hypoxia induced factor-1α(HIF-1α) expression in rats liver tissue were studied in this paper.Sixty male SD rats were randomly divided into 6 groups:(1) control group(C);(2) 8h hypoxic exposure group(8hHE);(3) 12h hypoxic exposure group(12hHE);(4) purely training group(T);(5) 8h HiLo group(8hHL);(6) 12h HiLo group(12hHL).Hypoxia chamber was simulated as altitude of 4000m.Groups T,8hHL and 12hHL were trained at the speed of 25m/min for 1 hour each day,5 days per week for 4 weeks.Immunohistochemical and computer image processing techniques were applied to detect the HIF-1α expression and its location in rats liver tissue.As compared with the group C,the expression of HIF-1α protein in groups 8hHE,12hHE,T,and both 8hHL and 12hHL increased significantly(P<0.05).There was no statistical significance between group 8hHL and group 12hHL(P>0.05).As compared with the groups 8hHE and 12hHE,the expression of HIF-1α protein in groups 8hHL and 12hHL increased significantly(P<0.05).As compared with the group T,the expression was even more significant in groups 8hHL and 12hHL(P<0.01).It was concluded that purely hypoxic exposure or purely training or HiLo could increase the expression of HIF-1α protein,but the effect of HiLo was more significant than that of purely hypoxic exposure or purely training.