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CONSTRUCTION AND IMMUNOGENICITY OF HUMAN PAPILLOMAVIRUS TYPE 6B L1 RECOMBINANT PLASMID
Authors:Fang Liu  Jia-bi Wang  Ya-gang Zuo  Yue-hua Liu  Dong-lai Ma
Institution:Department of Dermatology, Beijing Chaoyang Hospital, Beijing, 100020. roseliufang@sohu.com
Abstract:Objective To construct a DNA vaccine as a prophylactic model to prevent condyloma acuminatum and detect its immunogenicity in mice.Methods The major capsid protein (L1) gene of human papillomavirus (HPV) 6b was inserted into an eukaryotic expression plasmid (pcDNA3.1). The recombinant plasmid was transfected into COS-7 cells. Western blot were performed to detect whether L1 protein can be expressed in eukaryotic cells. Eighteen female BALB/c mice were tested for immunogenicity study.Results The recombinant plasmid (pcDNA3.1-HPV6bL1) was verified as HPV6b L1 gene by sequencing. Western blot showed specific strip. Anti-L1 protein antibodies could be detected in the mice's sera inoculated with pcDNA3.1-HPV6bL1.Similarly, IL-4, IL-2, and IFN-γ were increased in the same mice.Conclusion HPV6b L1 recombinant plasmid was constructed successfully which had immunogenicity for BALB/c mice. It provided experimental evidence for the research of DNA vaccine of condyloma acuminata.
Keywords:condyloma acuminata  human papillomavirus  DNA vaccine  RECOMBINANT PLASMID  TYPE  HUMAN PAPILLOMAVIRUS  IMMUNOGENICITY  provided  experimental  evidence  research  condyloma acuminata  increased  sera  antibodies  specific  strip  sequencing  Results  study  female  BALB  Western
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