胎鼠脊髓对成鼠急性脊髓损伤的修复作用——光镜CB—HRP示踪与还原银染色

把Ｅ１４胎鼠脊髓植入成鼠损伤脊髓后，分别在术后７、 １５、 ３０、 ４５、 ６０、 １２０天，用 ＣＢ－ＨＲＰ和还原银染色显示了宿主脊髓和移植物内的神经元及神经纤维。结果表明，虽然术后７天就可见宿主神经纤维再生进入移植物，但两种方法均不能显示移植的胚胎神经元及其突起。自术后３０天起，移植物内神经元可被标志，但神经元的体积小，突起数目也短而少。随着神经元体积的逐渐增大和突起数目的不断增多延长，可见宿主神经元向移植物内投射突起，并和后者神经元相互联系。作者认为，胚胎脊髓虽可在术后早期机械修补成鼠脊髓损伤，但并不能与宿主脊髓组织发生广泛的有机纤维联系，这和胚胎脊髓移植后，神经元的缓慢分化发育有关。

Function of fetal spinal cord on the repair of acute injured adult rats spinal cord: CB--HRP tracing and silver stain

After fetal spinal cord (E14) had been transplanted into adult rats spinal cord which had been hemisected and stired, neurons and nerve fibers of the host spinal cord and the grafts was studied by CB-HRP tracing and silver stain at day 7, 15, 30, 45, 60, 120 after operation. The results showed that although the host nerve fibers could regenerat into the grafts at day 7 after operation, fetal neurons and its processed couls not be showed by CB-HRP and silver. From day 30 on after transplantation, fetal neurons could be showed by CB-HRP, but its volume was little, its processes were shorter and fewer too. Afterwards as volume of fetal neurons enlarging as well as its processes increasing and prolonging gradually, the host neurons projected processes into the grafts and connected with the fetal neurons. We suggest that although embryonic spinal cord can repair the injured spinal cord mechanically at early stage after transplantation, they can not connect with host spinal cord organically. This may be related to the continuous growth of fetal neurons after transplantation.