HIF-1α、MMP-2的表达与膀胱移行细胞癌临床生物学行为的关系

目的探讨缺氧诱导因子1α（HIF—1α）、基质金属蛋白酶-2（MMP-2）在膀胱移行细胞癌（BTCC）组织中的表达及其与临床生物学行为之间的关系。方法应用免疫组织化学检测BTCC组织中HIF-1α和MMP-2的蛋白表达，分析其表达与肿瘤部分临床病理特点的关系，并分析二者表达的相关性。结果正常膀胱组织中均未见HIF—1α和MMP-2蛋白表达，膀胱移行细胞癌组织中HIF—lα和MMP-2蛋白的阳性表达率分别为52．63％（30／57）和49．12％（29／57）；HIF—lα阳性表达与肿瘤的临床分期及复发明显相关；MMP-2的阳性表达与肿瘤的病理分级、临床分期显著相关；HIF—lα的表达与MMP-2的表达密切相关。结论H1F—lα及MMP-2在BTCC的侵袭等生物学过程中具有重要的作用，HIF—lα和MMP-2也许可用于临床监控BTCC的进展。

Relationship Between the Expressions of HIF-1?and MMP-2 and the Biological Behavior in Human Bladder Transitional Cell Carcinoma

Objective To investigate the expressions of hypoxiainducibile factor lα（ HIF - lα） and matrix metalloproteinase 2 （ MMP - 2） protein and their relationship with the biological behavior in bladder transitional cell carcinoma（ BTCC）. Methods HIF- lα and MMP- 2 protein expression were determined using inmmnohistoche- mistty. The relationship between HIF - lα, MMP - 2 expression and various clinicopathological parameters including pathological grade and clinical stage was analyzed and the correlation between HIF- l ctexpression and MMP - 2 expression was also analyzed. Results No marked expression of HIF - lα and MMP - 2 was observed in the normal bladder tissues. The positive rate of HIF - lα and MMP - 2 in BTCC was 52.63% （30 of 57） and 49.12% （29 of 57） respectively. HIF- 1α protein expression was significantly associated with patients, TNM stage and relapse（ P 〈 0.05）. Significant differences existed in the expression of MMP - 2 in carcinomas from different pothological grades and clinical stages （P 〈 0,05）. The expression of HIF- lα protein was sig41ificantly correlated with MMP- 2 protein expression. Conclusion HIF- lα and MMP - 2 may play a critical role in the invasion of bladder carcinoma and may be used to estimate the progression of bladder carcinoma.