乳腺良恶性增生中Cyclin D1表达的研究

目的通过对乳腺良恶性增生中Cyclin D1蛋白表达的观察,研究Cyclin D1在乳腺良恶性增生中的表达状况及其在早期乳腺癌和良恶性乳腺肿瘤鉴别诊断中的价值.方法免疫组化LSAB法检测乳腺增生症、乳腺良性肿瘤、早期乳腺癌、乳腺浸润性导管癌中Cyclin D1蛋白表达.结果Cyclin D1蛋白在乳腺增生症中不表达;在乳腺良性肿瘤中表达率为7.5%;在乳腺恶性增生中表达阳性率为54.0%,其中早期乳腺癌为52.4%,浸润性导管癌为55.0%.Cyclin D1在早期乳腺癌,浸润性导管癌原发灶,腋窝淋巴结转移灶中表达阳性率无显著差异,三者阳性率分别为52.4%,55.0%,53.3%.结论Cyclin D1的蛋白表达在乳腺的良恶性增生之间的表达差异有显著性,可以应用于早期乳腺癌的辅助诊断.

PROTEIN EXPRESSION OF Cyclin D1 IN BREAST BENIGN AND MALIGNANT HYPERPLASIA

Objective:To detect protein expression of Cyclin D1 in breast benign hyperplasia and malignant hyperplasia.To discuss the value of Cyclin D1 detected by immunohistochemistry in the diagnosis of the early breast carcinoma.Methods:Detect protein expression of Cyclin D1 in hyperplasia of mamary glands,breast benign tumor,early breast carcinoma and infiltrative ductal breast carcinoma by immunohistochemistry.Results:Cyclin D1 protein was not expressed in hyperplasia of mamary glands.The expression rate of Cyclin D1 protein was 7.5% in breast benign tumor and was 54.0% in breast malignant hyperplasia.The positive rate was respectively 52.4% and 55.0% in carcinoma in situ and invasive ductal carcinoma.The positive rate of Cyclin D1 expression in early breast carcinoma,primary invasive ductal carcinoma and lymph node metastasis loci was respectively 52.4%,55.0%,53.3%.There was no significant difference among them.Conclusion:there was significant difference between breast benign and malignant hyperplasia,the detection of Cyclin D1 protein by immunohistochemistry could be used in the diagnosis of the breast carcinoma.