| HPLC法测定天元颗粒中天麻素的含量 |
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| 引用本文： | 冯瑛,夏正燕,徐强. HPLC法测定天元颗粒中天麻素的含量[J]. 中药新药与临床药理, 2009, 20(4) |
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| 作者姓名： | 冯瑛 夏正燕 徐强 |
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| 作者单位： | 浙江省中药研究所 杭州 310023 |
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| 摘 要： | 目的 测定天元颗粒中天麻素的含量.方法 样品采用甲醇回流提取,经中性氧化铝柱用80%乙醇洗脱收集;色谱柱:Diamonsil C18柱(250 mm×4.6mm,5μm),流动相:乙腈-0.15%磷酸水溶液(1:99),检测波长:220 nm,流速:1 mL/min,柱温:30℃.结果 天麻素在0.376～2.632 μg之间线性关系良好,r=0.9999 95(n=7),平均回收率99.09%,RSD为1.5%(n=6).结论 该法准确、重复性好,可用于天元颗粒制剂及天麻药材的含量测定及质量控制.
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| 关 键 词： | 天元颗粒 天麻素 |
Determination of Gastrodin in Tianyuan Granula by HPLC |
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| FENG Ying,XIA Zhengyan,XU Qiang. Determination of Gastrodin in Tianyuan Granula by HPLC[J]. Traditional Chinese Drug Research & Clinical Pharmacology, 2009, 20(4) |
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| Authors: | FENG Ying XIA Zhengyan XU Qiang |
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| Abstract: | Objective To establish a method for the determination of gastrodin in Tianyuan Granula. Methods The sample was refluxed with methanol, then eluted with 80 % ethanol through the alumina column(neutral). The chromato-graphic conditions were as follows: Diamonsil Cts chromatographic column(250 mm ×4.6 mm, 5 μm) with a mobile phase of acetonitrile-0. 15 % phosphoric acid (1 : 99), the detection wavelength being at 220 nm and the flow rate be-good (r=0.9999 95, n=7).The average recovery was 99.09 % and RSD=1. 50 % (n=6). Conclusion The method for determination of gastrodin in Tianyuan Granula is accurate, and with a good reproducibility. |
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| Keywords: | HPLC Tianyuan Granula Gastrodin HPLC |
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