| 辛德毕斯病毒结构蛋白基因对宿主细胞影响的初步研究 |
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| 引用本文： | 李蕾,梁国栋,赵炳文,周国林,付士红,何海怀,金奇,侯云德.辛德毕斯病毒结构蛋白基因对宿主细胞影响的初步研究[J].中华实验和临床病毒学杂志,2000,14(3):218-222. |
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| 作者姓名： | 李蕾 梁国栋 赵炳文 周国林 付士红 何海怀 金奇 侯云德 |
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| 作者单位： | 100052 北京，中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室 |
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| 基金项目： | 国家自然科学基金资(39970037) |
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| 摘 要： | 目的 了解辛德毕斯病毒基因结构与功能的关系，阐明其分子致病分子机理。方法 构建了辛德毕斯病毒（ＸＪ－１６０病毒株）结构基因的真核表达质粒，ｐｃＤＮＡ３．１ＡＢＣ，转染ＢＨＫ－２１细胞后观察其对宿主细胞的影响。结果 转染病毒结构基因的ＢＨＫ－２１细胞，可以发生辛协毕斯病毒感染所出现的细胞毒性反应，表现为：细胞存活率降低；细胞核染色质浓缩、凝集；Ｇ１期细胞减少、而处于Ｓ期的细胞增多。结论 辛德毕斯病毒
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| 关 键 词： | 辛德毕斯病毒 脱噬作用 结构基因 |
| 修稿时间： | 1999年9月23日 |
Effect of Sindbis XJ- 160 virus structural proteins on BHK-21 cell |
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| LI Lei.Effect of Sindbis XJ- 160 virus structural proteins on BHK-21 cell[J].Chinese Journal of Experimental and Clinical Virology,2000,14(3):218-222. |
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| Authors: | LI Lei |
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| Institution: | State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052, China. |
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| Abstract: | Objective To study the effect of Sindbis XJ-160 virus structural proteins on BHK-21 cell. Methods We constructed the expressing vector pcDNA3.1ABC that contains the XJ-160 virus structural protein gene,and then transfected it to BHK-21 cell.Results The expressed products can be detected by RNA dot blot hybridization or immnofluorescent assay.The cytopathic effect was observed in BHK-21 cells transfected with pc DNA3.1ABC. There was also condensed chromatin found accumulated.BHK-21 cell death and marked cell cycle changes were observed. Conclusions These results indicate that cell appoptosis might be one of the mechanisms inducing cell death by XJ-160 virus and its structural protein gene probably plays a role in this programmed cell death. |
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| Keywords: | Sindbis virus Apoptosis Structural gene |
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