Purification of Human Basophils by Affinity Chromatography on Anti-IgE-Sepharose 6MB |
| |
| Authors: | J. B. C. Toll J. E. S. Wikberg R. G. G. Andersson |
| |
| Affiliation: | Department of Pharmacology, Linköping University, Linköping, Sweden |
| |
| Abstract: | This work describes a method for the purification of basophil leukocytes from human peripheral blood by the use of a three-step separation technique including affinity chromatography on anti-IgE-sepharose 6MB. The purity of the obtained basophils was 50–95% and the recovery was 30–40%. The basophils separated by this method appeared normal and were found to be reactive with anti-IgE in subsequent tests. |
| |
| Keywords: | basophils purification |
|
|
