| 造血干细胞分化过程中HOXB6基因表达及干预研究 |
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| 引用本文： | 陈红英,陈艾,刘文君.造血干细胞分化过程中HOXB6基因表达及干预研究[J].实用儿科临床杂志,2010(3). |
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| 作者姓名： | 陈红英 陈艾 刘文君 |
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| 作者单位： | 泸州医学院附属医院儿科; |
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| 基金项目： | 四川省教育厅重点科研项目(2004A058) |
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| 摘 要： | 目的探讨脐血造血干细胞(HSC)向粒-单系(CFU-GM)、红系(CFU-E)和淋巴系祖细胞(CFU-TL)增殖分化过程中HOXB6基因的表达情况及全反式维A酸(ATRA)对其的影响。方法1.采用造血干/祖细胞体外培养技术,以ATRA持续干扰人类脐血造血干/祖细胞,观察HSC经人粒细胞-单核细胞集落刺激因子(GM-CSF)、促红细胞生成素(EPO)和植物血凝素(PHA)分别诱导后,在培养第3天、第7天和第12天的CFU-GM、CFU-E及CFU-TL集落生成情况。2.采用实时荧光定量RT-PCR技术(FQ-RT-PCR)检测造血干细胞增殖分化过程中HOXB6基因的表达水平。结果1.人类造血干细胞向CFU-GM、CFU-E和CFU-TL增殖分化过程中,各组细胞HOXB6基因均有表达。2.随时间推移,CFU-GMHOXB6 mRNA在增殖分化的第3天开始表达,第7天表达最强烈,第12天表达明显减弱;CFU-E HOXB6 mRNA表达在第3天、第7天和第12天均持续增强;而在CFU-TL中,HOXB6 mRNA表达在第7天最强烈,第12天表达减弱。3.与对照组比较,ATRA可上调各组HOXB6基因的表达。结论1....
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| 关 键 词： | HOXB6基因 造血干细胞 实时荧光定量反转录聚合酶链反应 |
Expression of HOXB6 Gene on Differentiation of Hematopoietic Stem Cells and Intervention Research |
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| CHEN Hong-ying,CHEN Ai,LIU Wen-jun.Expression of HOXB6 Gene on Differentiation of Hematopoietic Stem Cells and Intervention Research[J].Journal of Applied Clinical Pediatrics,2010(3). |
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| Authors: | CHEN Hong-ying CHEN Ai LIU Wen-jun |
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| Institution: | CHEN Hong-ying,CHEN Ai,LIU Wen-jun (Department of Pediatrics,the Affiliated Hospital of Luzhou Medical College,Luzhou 646000,Sichuan Province,China) |
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| Abstract: | Objective To observe the expression level of HOXB6 gene on the differentiation and proliferation of hematopoietic stem cells(HSC) to colony forming unit-granulocyte-monocyte(CFU-GM),erythroid progenitor(CFU-E) and colony forming unite-T-lymphocyt(CFU-TL).And the proliferation procress was affected by all-transretinoic acid(ATRA).Methods 1.By the colony culture in vitro,the impact of ATRA on the CFU-GM,CFU-E,CFU-TL colony formation were surveyed;the expressions of HOXB6 gene were observed on the differentiat... |
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