| HLA-DRB1等位基因分型与类风湿关节炎相关性研究 |
| |
| 引用本文： | 蒋真,李向培,钱龙,厉小梅,汪国生. HLA-DRB1等位基因分型与类风湿关节炎相关性研究[J]. 安徽医药, 2010, 14(11): 1299-1302. DOI: 10.3969/j.issn.1009-6469.2010.11.024 |
| |
| 作者姓名： | 蒋真 李向培 钱龙 厉小梅 汪国生 |
| |
| 作者单位： | 南京医科大学附属淮安市第一人民医院免疫风湿科,江苏,淮安,223300;安徽医科大学附属省立医院风湿科,安徽,合肥,230022 |
| |
| 基金项目： | 安徽省自然科学基金资助项目 |
| |
| 摘 要： | 目的探讨HLA-DRB1等位基因在皖籍江淮地区类风湿关节炎患者的分布及意义。方法采用SSP（序列特异性引物）多重PCR技术扩增106例RA患者和340例正常对照HLA-DRB1等位基因,先对HLA-DRB1位点进行低分辨率分型,再进一步对低分亚型HLA-DRB1＊04,DRB1＊10,DRB1＊01和DRB1＊14做高分辨率分型。计算并比较两组等位基因携带率。结果低分结果共13个亚型,其中HLA-DRB1＊04,DRB1＊09和DRB1＊10在RA组的频率显著高于正常对照组（P=0.005,0.039,0.000）HLA-DRB1＊11,DRB1＊15和DRB1＊07的频率显著低于对照组（P=0.005,0.036,0.001）。HLA-DRB1＊01,DRB1＊14在两组差别无显著意义（P=0.432和P=0.359）。高分结果HLA-DRB1＊0405和DRB1＊1001在RA组的频率显著高于正常对照组（P=0.001和P=0.000）。结论 HLA-DRB1位点在皖籍江淮地区类风湿关节炎患者中的分布具有丰富的多态性,共同表位等位基因（SE等位基因）HLA-DRB1＊0405、DRB1＊1001及非SE等位基因HLA-DRB1＊09可能是皖籍江淮地区类风湿关节炎患者的易感基因,HLA-DRB1＊11,DRB1＊15和DRB1＊07可能是保护性等位基因。
|
| 关 键 词： | 关节炎 类风湿 HLA-DRB1基因 等位基因 共同表位 |
Different effects of HLA-DRB1 alleles on RA |
| |
| JIANG Zhen,LI Xiang-pei,QIAN Long,LI Xiao-mei,WANG Guo-sheng. Different effects of HLA-DRB1 alleles on RA[J]. Anhui Medical and Pharmaceutical Journal, 2010, 14(11): 1299-1302. DOI: 10.3969/j.issn.1009-6469.2010.11.024 |
| |
| Authors: | JIANG Zhen LI Xiang-pei QIAN Long LI Xiao-mei WANG Guo-sheng |
| |
| Affiliation: | JIANG Zhen1,LI Xiang-pei2,QIAN Long2,LI Xiao-mei2,WANG Guo-sheng2(1.Department of Rheumatology ﹠ Immunology,The First Affiliated People’s Hospital of Huaian,Nanjing Medical University,Huaian 223300,China;2.Provincial Hospital,Anhui Medical University,Hefei,Anhui 230022,China) |
| |
| Abstract: | Aim To explore the distribution of HLA-DRB1 alleles in RA(rheumatoid arthritis) patients of Anhui province and to investigate the association between HLA-DRB1 alleles and RA patients.Methods Multiple sequence specific primer PCR(SSP-PCR) was used to amplify HLA-DRB1 alleles.First,the method of low resolution SSP-PCR was used to amplify HLA-DRB1 alleles;second,the method of high resolution SSP-PCR was used to amplify HLA-DRB1*04,DRB1*10,DRB1*01 and DRB1*14 subtypes;next,allele carriage rates between RA patie... |
| |
| Keywords: | arthritis rheumatoid HLA-DRB1 gene allele shared epitope |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
