| 大鼠骨髓间充质干细胞体外向神经元样细胞的诱导 |
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| 引用本文： | 谷艳娇,李博,高志安.大鼠骨髓间充质干细胞体外向神经元样细胞的诱导[J].中国组织工程研究与临床康复,2010,14(14). |
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| 作者姓名： | 谷艳娇 李博 高志安 |
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| 作者单位： | 辽宁医学院病理学教研室,辽宁省锦州市,121001 |
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| 摘 要： | 背景:选择合适的诱导方法诱导骨髓间充质干细胞为神经元样细胞是临床治疗神经损伤的关键.目的:在大鼠骨髓间充质干细胞中加入大鼠齿状回蛋白提取物,观察其诱导分化为神经元样细胞的可行性.方法:应用20,40,60,80 mg/L齿状回蛋白提取物诱导分化大鼠骨髓间充质干细胞,蛋白印记检测神经元分化情况,筛选最佳剂量.收集诱导后的神经元样细胞,倒置显微镜下观察分化后细胞的形态学变化,用蛋白印记技术检测NSE、NeuN的表达,免疫荧光技术检测细胞内NeuN的表达.结果与结论:诱导分化7d后,经蛋白印记检测可见60mg/L的齿状回蛋白提取物为最佳诱导剂量,随质量浓度的增加NSE与NeuN的表达量逐渐增加,当质量浓度为80 mg/L时,细胞表达的NSE与NeuN减少;选择60 mg/L齿状回蛋白提取物诱导分化骨髓间充质干细胞,诱导后的细胞变长,有突触:免疫荧光NeuN染色发现,诱导分化后的神经元样细胞有阳性表达.结果提示齿状回提取物是一种可以诱导骨髓间充质干细胞分化的有效生物诱导剂.
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| 关 键 词： | 齿状回提取物 骨髓间充质干细胞 诱导分化 神经元样细胞 生物诱导剂 |
Induction and differentiation of rat bone marrow mesenchymal stem cells into neuron-like cells in vitro |
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| Gu Yan-jiao,Li Bo,Gao Zhi-an.Induction and differentiation of rat bone marrow mesenchymal stem cells into neuron-like cells in vitro[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(14). |
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| Authors: | Gu Yan-jiao Li Bo Gao Zhi-an |
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| Institution: | Gu Yan-jiao,Li Bo,Gao Zhi-an Department of Pathology,Liaoning Medical University,Jinzhou 121001,Liaoning Province,China |
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| Abstract: | BACKGROUND:It is a key to choose an appropriate method to trans-differentiated bone marrow mesenchymal stem cells(BMSCs) into neuron-like cells for clinical therapy of neural system injury.OBJECTIVE:To observe the feasibility of the differentiation of rat BMSCs supplemented with rat dentate gyrus extract(DGE) into neuron-like cells.METHODS:DGE was applied to induce rat BMSC trans-differentiation,using 20,40,60,80 mg/L protein concentration.Neuron differentiation was measured using Western blot to screen an ... |
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