太空环境诱变黑色素瘤细胞的实验研究

目的观察太空环境对B16细胞生物学特性的影响，寻找免疫原性增强的细胞株。方法将小鼠黑色素瘤B16细胞搭载于第20颗返回式卫星，返地后单克隆化，选择1株形态变异的单克隆细胞株（1^#），MTT法检测细胞增殖情况，接种C57BL／6J小鼠，分别观察细胞致瘤性、荷瘤小鼠免疫器官、生存期及瘤体病理变化。结果1^#太空诱变细胞株，形态呈现树突状，增殖速率与对照细胞比较差异无统计学意义（P〉0．05），接种C57BL／6J小鼠，肿瘤潜伏期延长（P〈0．01），瘤体重量减小（P〈0.01），胸腺系数增大（P〈0．01），且荷瘤小鼠生存期延长（P〈0，01），而脾系数与对照组比较差异无统计学意义（P〉0．05），病理学结果显示：1^#细胞组瘤体内血管不及对照组丰富，坏死区较少，瘤体内浸润淋巴细胞较对照组有不同程度的增多，瘤旁组织内有较多淋巴细胞。结论空间诱变1^#细胞株可能趋向分化，体内实验结果提示：1^#诱变细胞株抗原性可能被增强，激发了小鼠的免疫应答，该结果为进一步筛选免疫原性增强的细胞株，确定抗原的强化特征提供了实验基础。

Study on melanoma cell lines mutated in outer space environment

Objective To study the mutagenic effects of outer space environment on biological characteristics of melanoma B16 cell lines and screen the immunogenicity-enhanced cell line. Methods The murine melanoma B16 cells were carried in the No.20 return satellite and the survived cells were monocloned. One monoclonal cell line(1#), which morphology varied, was utilized for further study. MTT assay was used for analysis of cell proliferation. The tumor free time, immune organ weight and survival time were observed after subcutaneous or abdominal injection of the cells to C57BL/6J mice and the tumor lumps were examined by routine pathology. Results Compared with the control group, the morphology of 1# cell line showed dendritic form and its growth rate was insignificant as analyzed by SSPS . The tumor_free and survival times were both prolonged and the weights of tumors were reduced. The thymus gland coefficient was increased for 1# cell line. Simultaneously with that, the lymphocyte infiltration was augmented both in tumor and adjacent tissues in mice injected with 1# cells. Conclusions The 1# cell line,which was mutated in outer space environment, was trending towards differentiation. The present data imply that the immunogenicity of 1# cell line might be enhanced. Our observations could provide insights into the further screen of immunogenicity_enhanced cell lines.;