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HPLC法测定尿素氢化可的松乳膏中氢化可的松的含量
引用本文:陈笑天,刘慧莹,刘小英,詹姿女,徐东峰,李斌. HPLC法测定尿素氢化可的松乳膏中氢化可的松的含量[J]. 江西中医学院学报, 2011, 23(5): 69-70
作者姓名:陈笑天  刘慧莹  刘小英  詹姿女  徐东峰  李斌
作者单位:江西中医学院 南昌 330004
摘    要:目的:建立测定尿素氢化可的松乳膏中氢化可的松含量的方法。方法:采用高效液相色谱法。色谱柱为Hypersil ODS(4.6 mm×250 mm,5μm);流动相为甲醇-水(50:50),流速为1.0 ml/min,氢化可的松的检测波长为245 nm,柱温为30℃,进样量为20μl。结果:氢化可的松在3.15~22.05μg/ml(r=0.999 9)的范围内,线性关系良好。平均回收率为99.8%(RSD=1.74%)。结论:方法快速灵敏、结果准确,可用于氢化可的松乳膏的质量控制。

关 键 词:氢化可的松  高效液相色谱法  含量测定

Content Determination of Hydrocortisone in the Compound Urea and Hydrocortisone Cream
CHEN Xiao-tian,LIU Hui-ying,LIU Xiao-ring,ZHAN Zi-nv,XU Dong-feng,LI Bin. Content Determination of Hydrocortisone in the Compound Urea and Hydrocortisone Cream[J]. Journal of Jiangxi College of Traditional Chinese Medicine, 2011, 23(5): 69-70
Authors:CHEN Xiao-tian  LIU Hui-ying  LIU Xiao-ring  ZHAN Zi-nv  XU Dong-feng  LI Bin
Affiliation:CHEN Xiao-tian,LIU Hui-ying,LIU Xiao-ying,ZHAN Zi-nv,XU Dong-feng,LI Bin* Jiangxi University of Traditional Chinese Medicine,Jiangxi Nanchang 330004
Abstract:Objective:To establish the method for the content determination of Hydrocortisone in the Compound Urea and Hydrocortisone Cream.Methods: HPLC method was adopted.The column was Hypersil ODS(4.6 mm×250 mm,5 μm) with the mobile phase consisted of methanol-water(50:50) at flow rate of 1.0 mlμmin-1.The detection wavelength was set at 245nm,the injection volume was 20μl and column temperature was 30℃.Results: The linear range of hydrocortisone was 3.15~22.05 μg/ml(r=0.9999).The average recoveries was 99.8%(RSD=1.74%).Conclusion: The method is simple,rapid,sensitive and suitable for the content determination of hydrocortisone in the hydrocortisone cream.
Keywords:Hydrocortisone  HPLC  Content determination  
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