HPLC-ELSD同时测定山银花中绿原酸、木犀草苷和川续断皂苷乙的含量

目的:建立高效液相色谱-蒸发光散射检测法测定山银花中绿原酸、木犀草苷和川续断皂苷乙的方法。方法:采用Col-umn XB-C18柱(250 mm×4.6 mm,5μm)为色谱柱,用乙腈(A)-0.5%冰醋酸水溶液(B)为流动相梯度洗脱,以蒸发光散射检测器检测绿原酸和川续断皂苷乙。梯度程序为:0 min(A:9%,B:91%)保持10→12 min(A:29%,B:71%)→20 min(A:33%,B:67%)→25 min(A:45%,B:55%),流速1.0 ml/min;检测器漂移管温度100℃,载气流速为3.0 ml/min。结果:绿原酸、木犀草苷及川续断皂苷乙的回归方程分别为:Y绿原酸=1.341 1X+4.360 1,r=0.999 5;Y木犀草苷=25 948.31X-22.863 41,r=0.999 92,Y川续断皂苷乙=5.688 8 X+5.350 6,r=0.999 6。线性范围分别为:绿原酸0.005-0.045 mg/ml;木犀草苷0.017 7-0.085 5 mg/ml,川续断皂苷乙0.048 6-0.243 mg/ml。结论:该方法操作简便、结果准确,专属性强,分离效果和重现性好,可有效缩短总分析时间,为较全面控制金银花药材的质量控制提供了简便可靠的方法。

HPLC-ELSD Determination of Chlorogenic Acid、 Galuteolin And Dipsacoside B in Flos Lonicerae Confusae

Objective： To develop a method for determination of Chlorogenic Acid,Galuteolin And Dipsacoside B in Flos Lonicerae Confusae.Methods：An HPLC equipped with ELSD method was applied to quantify chlorogenic Acid,Galuteolinand Dipsacoside B.The analytical column was Column XB-C18（250 mm×4.6 mm,5μm）with the temperature of30℃.Two solution were acetonitrile（A）and 0.5% acetic acid solution and the gradient mode was as follows：0min（A：9%,B：91%）（maintain 10 min）→12 min（A：29%,B：71%）→20 min（A：33%,B：67%）→25 min（A：45%,B：55%）,The flow rate was 1.0ml/min.The temperature of drift tube was set at 100℃ and the flow rate of gas maintained at 3.0ml/min.Results： The calibration curves of Chlorogenic Acid,Galuteolin And Dipsacoside B were linear in the range of 0.005-0.045mg/m,0.0177-0.0855mg/ml and 0.0486-0.243mg/ml.Conclusion： The method is accurate,specific,and can shorten the total analytical time of Flos Lonicerae.