乙酰肝素酶和血管内皮生长因子C mRNA的表达与肺癌侵袭和转移的分子生物学

目的研究乙酰肝素酶(HPSE)和血管内皮生长因子(VEGF)-C mRNA的表达是否与肺癌的侵袭性生长和淋巴结转移有关。方法应用RT-PCR技术检测HPSE和VEGF-C mRNA在65例肺癌组织中的表达,并与癌旁组织和正常肺组织对比,同时结合肺癌的临床病理学特征及预后进行分析。结果肺癌组织中HPSE和VEGF-C mRNA的阳性表达率明显高于正常肺组织和癌旁组织(P<0.05);不同病理类型肺癌组织中HPSE和VEGF-C蛋白阳性表达率均无显著性差异(P>0.05);不同分化程度肺癌组织中HPSE和VEGF-C蛋白阳性表达率均无显著性差异(P>0.05);Ⅲ期和Ⅳ期肺癌组织中HPSE和VEGF-C蛋白阳性表达率明显高于Ⅰ期和Ⅱ期(P<0.05);生存3年以下的肺癌组织中HPSE和VEGF-C蛋白阳性表达率明显高于生存3年以上者(P<0.05)。结论肺癌的发生、发展可能与HPSE促血管生成和VEGF-C促淋巴管生成的协同作用有关;HPSE和VEGF-C基因表达可作为判断肺癌生物学行为和患者预后的一个参考指标。

Study of HPSE and VEGF-C mRNA expressions with invastveness and metastasis in human lung cancer

Objective To study the expressions of HPSE and mRNA with invasiveness and metastasis in human lung cancer.Methods Expressions of HPSE and VEGF-C mRNA in 65 cases of lung cancer,adjacent tissues of cancer and normal tissues were tested by RT-PCR and analyzed by clinicopathological characteristics and prognosis of lung cancer.Results The positive rate of HPSE and VEGF-C mRNA in tumor tissues were significantly higher than those in adjacent tissues of cancer and normal tissues(P<0.05).There was not significant difference between HPSE and VEGF-C mRNA expressions and the type and grade of the tumor(P>0.05).But the expressions of HPSE and VEGF-C mRNA were correlated with the clinical stage and survival time of the patients(P<0.05).Conclusions Overexpressions of HPSE and VEGF-C mRNA in lung cancer tissues play role in occurrence and development of lung cancer.The expression of HPSE and VEGF-C mRNA can be used as an useful marker of the biological behavior of lung cancer and an independent prognosis factor for the patients with lung cancer.