白花蛇舌草对荷瘤鼠脾脏淋巴细胞影响的实验研究

目的：探讨白花蛇舌草对荷瘤鼠电离辐射后脾淋巴细胞结构和功能的影响。方法：将U14宫颈癌细胞系种植建立荷瘤鼠模型，分单纯照射组和照射＋中药组，分别应用透视电镜观察鼠脾脏淋巴细胞超微结构变化，流式细胞仪检测淋巴细胞增殖和细胞周期变化，脾各淋巴细胞亚群对U14细胞攻击能力的变化。结果：荷瘤鼠经全身照射同时给以白花蛇舌草灌胃可减轻荷瘤鼠脾淋巴细胞器损伤，促进淋巴细胞增殖，并提高各亚群攻击U14细胞能力。结论：白花蛇舌草可通过保护脾淋巴细胞提高荷瘤鼠免疫能力达到其抗电离辐射效应。

Study on Effect of Hedyotic Diffusa on Spleen Lymphocytes in Tumor-Bearing Mices Treated with Radiotherapy

Objective To study the effect of hedyotic diffusa on spleen lymphocytes in tumor - bearing mices treated with radiotherapy. Methods Electron microscope was used to observed ultrastructure of spleen. The proliferation and S phase of spleen lymphocytes were determined with flow cytometry and the natural killing activity were determined by means of 3 H - TdR release assay. Resuits Hedyotic diffusa could ameliorate spleenic ultrastructural injury in tumor - bearing mices treated with radiotherapy, accelerate proliferation of spleen lymphocytes, and enhance their capacity to kill U14 cells. Conclusion Hedyotic diffusa provided protection against radioactive injury for spleenic immunocytes in experimental mice.