成纤维细胞-无细胞真皮替代物的生物学活性及移植实验

目的 研究含成纤维细胞的无细胞真皮替代物的生物学活性及真皮支架作用。方法 将成纤维细胞种植于无细胞真皮表面培养，形成活性真皮替代物。采用ELISA法和RIA法测定培养上清中IL－6、IL－8、TGF－β1及细胞外基质层粘连蛋白、透明质酸的分泌。并将真皮替代物植入BALB／c-nu小鼠（裸鼠）全层皮肤缺损创面，观察血管化速度、创面收缩率。结果 成纤维细胞种植于无细胞真皮表面生长良好，可形成单层细胞膜片，并分泌多种细胞因子和细胞外基质成份。活性真皮替代物植入创面后，与单纯无细胞真皮移植相比，血管化速度加快，收缩率减小。结论 无细胞真皮上种植成纤维细胞后具有较强的生物学活性，可作为较好的真皮替代物。

Bioactivity and test grafting of acellular dermal matrix containing fibroblasts

Objective To investigate the bioactivity of acellular dermal matrix with fibroblasts and its role as dermal skeleton. Methods Human fibroblasts( HFs) were planted onto the surface of acellular der- mal matrix ( ADM) to form living dermal substitute. The 1L - 6, IL - 8 and TGF contents in the supernatant of the culture of HF - ADM were determined with ELISA method, and the secretion of hyaluronic acid and laminin from extracellular matrix was measured with RIA method. The speed of vascularization and the wound contracture rate were observed after the dermal substitute was grafted on the full skin loss wound of Balb/c - nu mice (nude mice). Results HFs grew very well after being planted onto ADM so as to form a single layer of celluar membrane. Many kinds of eytokines and extracelluar matrix components were secreted. Com- pared with simple acellular dermal grafting, the vascularization was accelerated, and the wound contracture rate decreased, after the living dermal substitute being grafted on the wound. Conclusion The ADM see- ded with HFs exhibited excellent bioactivity and might be an optimal dermal substitute.