不同方法提取薰衣草挥发油主要成分的GC测定

目的 GC测定不同方法提取薰衣草挥发油中芳樟醇和乙酸芳樟酯的含量。方法采用毛细管气相色谱法,以间二硝基苯为内标,采用DB-5弹性石英毛细管柱（30 m×0.25 mm,0.25μm）,FID检测器;进样口温度为：250℃,分流比20∶1,流量：1.0 mL.min 1,检测器温度：220℃;程序升温：110℃保持3 min,以10℃.min 1升至130℃保持4 min,然后以20℃.min 1升至250℃保持1 min。结果在该色谱条件下,芳樟醇、乙酸芳樟酯及内标物间二硝基苯均得到良好的分离;测得超临界CO2萃取的精油中芳樟醇的含量为38.02%,乙酸芳樟酯的含量为34.24%,而通过水蒸气蒸馏法提取的精油中芳樟醇的含量为59.09%,乙酸芳樟酯的含量为18.14%。结论本方法简便、准确、重复性好,可用于不同提取方法提取的薰衣草挥发油的质量控制。

Content Determination of the Essential Oil from Lavender with Different Extraction Methods by GC

OBJECTIVE To determine the linalyl acetate and linalool content in the essential oil from lavender with different extraction methods by GC.METHODS The capillary GC system consisted of a DB-5 capillary column(30 m×0.25 mm,0.25 μm),and meta-dinitro-benzent was used as an internal standard.The detector was FID;inlet temperature was 230 ℃;split ratio was 20∶1;the flow rate was 1.0 mL·min-1;the detector temperature was 250 ℃.The incept temperature programmed was 110 ℃ and kept for 3 min;programmed from 110 ℃ to 130 ℃ at 10 ℃·min-1 and kept for 4 min,and then progammed to 250 ℃ at 20 ℃·min-1 and kept for 1 min.RESULTS Linalool,linalyl acetate and the internal standard were separated in the chromatographic condition.The linalool content from the supercritical CO2 extract in the volatile oil was 38.02%,the linalyl acetate content was 34.24%,the linalool content from the steam distillation extract in the volatile oil was 59.09%,the linalyl acetate content was 18.14%.CONCLUSION The method is accurate,selectivity,and can be used as quality control for the lavender oil extracted with different methods.