加工前、后的亳州牡丹皮水提物对2型糖尿病小鼠血糖、血脂等的作用研究

目的:研究加工前、后的亳州牡丹皮水提物对2型糖尿病（T2DM）小鼠的血糖（GLU）、总胆固醇（TC）、三酰甘油（TG）、超氧化物歧化酶（SOD）、丙二醛（MDA）和游离脂肪酸（NEFA）的作用比较。方法:采用高脂饲料喂养雄性昆明小鼠1个月,再采用链脲佐菌素（STZ）120 mg·kg^-1进行腹腔注射,复制T2DM小鼠模型,然后,随机分配已建立成功的小鼠模型至模型对照组、阳性对照组、未加工丹皮给药组和已加工丹皮给药组,并以正常小鼠作为正常对照组。对造模成功的小鼠以各丹皮水提物进行28 d的持续灌胃,再解剖取血,测定T2DM小鼠血清中的各项生化指标。结果:已加工牡丹皮和未加工牡丹皮水提物均能显著的降低T2DM小鼠体内的GLU（P<0.01）、TC（P<0.01）、MDA（P<0.05）,并能使T2DM小鼠体内的SOD显著升高（P<0.01）。结论:加工前、后的牡丹皮水提物均能显著降低T2DM小鼠的血糖值,两者差异无统计学意义,并对血脂的代谢和抗氧化的能力在一定程度上有调节作用。已加工的牡丹皮外观上虽然更加美观,但是在药效上并未增强。

Effects of Pre and Post processed Bozhou Cortex Moutan Water Extract on Blood Glucose, and Lipid in Type 2 Diabetic Mice

Objective： To study the effects of pre- and post-processed Bozhou cortex moutan aqueous extract on the blood glucose （GLU） , total cholesterol （ TC ）, triglycerides （ TG ）, superoxide dismutase （ SOD ）, malondialdehyde aldehyde （ MDA ） and free fatty acids （NEFA） in type 2 diabetic （T2DM）mice. Method： Kunming male mice were fed with high fat diet for a month followed by streptozotocin （STZ） 120 mg· kg^-1 intraperitoneal injection to obtain type 2 diabetic model, and then randomly divided into the control group,positive control group,the raw cortex moutan-treated group and processed cortex moutan-treated group with normal mice as the normal control. The mice in processed cortex moutan-treated group were orally treated by cortex moutan water extract for 28 days continuously, and then the blood samples were w＇ithdrawn to determine the serum biochemical indicators. Result： Processed and unprocessed cortex moutan aqueous extract both could significantly reduce GLU （P 〈0. 01 ） ,TC （P 〈0. 01 ） and MDA （P 〈0. 05） while increase SOD （ P 〈 0. 01 ） in type 2 diabetic mice. Conclusion ： Preand post-processed cortex moutan aqueous extract can significantly reduce the blood glucose levels in type 2 diabetic mice without significant difference, and adjust the lipid metabolism and antioxidant capacity to some extent. Cortex moutan processed exhibits more promising appearance, however does not enhance the efficacy.