| Hsa-miR-132通过靶向FOXA1基因影响食管癌细胞的增殖和侵袭 |
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| 引用本文： | 李书军,张秀金,侯俊峰,宋学平,李凤军,申富生,张合林.Hsa-miR-132通过靶向FOXA1基因影响食管癌细胞的增殖和侵袭[J].中国肿瘤生物治疗杂志,2011,18(5):485-489. |
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| 作者姓名： | 李书军 张秀金 侯俊峰 宋学平 李凤军 申富生 张合林 |
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| 作者单位： | 1. 河北医科大学第二医院胸外科,河北石家庄,050051
2. 河北省涉县医院胸外科,河北邯郸,056400 |
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| 基金项目： | 河北省卫生厅重点课题计划资助项目（No.20110068） |
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| 摘 要： | 目的:探讨hsa-miR-132(miR-132)对人食管癌细胞增殖和侵袭的影响及其可能的机制。方法:构建pCDNA3.1(+)-miR-132表达载体并转染高转移潜能的人食管癌细胞KYSE150,G418筛选稳定过表达miR-132的KYSE150细胞。软琼脂克隆形成实验和Transwell小室实验检测KYSE150细胞的增殖和侵袭,Real-time PCR和Western blotting检测miR-132和叉头框蛋白A1基因(forkhead box protens A1,FOXA1)在KYSE150细胞中的表达,双荧光素酶报告基因分析和Western blotting检测过表达miR-132对FOXA1的调控作用。结果:成功构建pCDNA3.1(+)-miR-132质粒,获得稳定过表达miR-132的KYSE150细胞。亲本KYSE150细胞中miR-132低表达,而FOXA1蛋白高表达。与转染空载体和未转染KYSE150细胞相比,转染pCDNA3.1(+)-miR-132不影响KYSE150细胞的增殖(13.9±0.33 vs 15.4±0.11、17.1±0.20,P>0.05),但细胞体积较小且表面比较光滑;其可显著降低KYSE150细胞的侵袭能力(55±1.6)vs(129.0±3.1)、(124.0±2.8)个细胞,P<0.01]。miR-132能够作用于FOXA1 3’-UTR,从而抑制内源性FOXA1的表达。结论:食管癌KYSE150细胞低表达miR-132,外源性过表达miR-132可负向调控FOXA1的表达,从而抑制KYSE150细胞的侵袭能力。
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| 关 键 词： | 食管癌 侵袭 增殖 has-miR-132 FOXA1基因 |
| 收稿时间： | 7/6/2011 12:00:00 AM |
| 修稿时间： | 2011/8/30 0:00:00 |
Hsa-miR-132 affects proliferation and invasion of esophageal carcinoma cells by targeting FOXA1 gene |
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| LI Shu jun,LI Xiu jing,HOU Jun feng,SONG Xue ping,LI Feng jun,SHE Fu sheng and ZHANG He lin.Hsa-miR-132 affects proliferation and invasion of esophageal carcinoma cells by targeting FOXA1 gene[J].Chinese Journal of Cancer Biotherapy,2011,18(5):485-489. |
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| Authors: | LI Shu jun LI Xiu jing HOU Jun feng SONG Xue ping LI Feng jun SHE Fu sheng and ZHANG He lin |
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| Institution: | Department of Thoracic Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China;Department of Thoracic Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China;Department of Thoracic Surgery, Shexian Hospital, Handan 056400, Hebei, China;Department of Thoracic Surgery, Shexian Hospital, Handan 056400, Hebei, China;Department of Thoracic Surgery, Shexian Hospital, Handan 056400, Hebei, China;Department of Thoracic Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China;Department of Thoracic Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei, China |
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| Abstract: | Objective:To investigate the effect of hsa-miR-132(miR-132) on proliferation and invasion of human esophageal carcinoma cells,and further explore its possible mechanism.Methods: pCDNA3.1(+)-miR-132 plasmid was constructed,and KYSE150 cells stablely overexpressing miR-132 were selected through G418 screening.Soft agar colony formation test and Transwell assay were performed to analyze the proliferation and invasion of KYSE150 cells.Expression of miR-132 and forkhead box protens A1(FOXA1) in KYSE150 cells wer... |
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| Keywords: | esophageal carcinoma invasion proliferation hsa-miR-132 FOXA1 |
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