灵芝提取物抗肿瘤作用的实验研究

目的探讨一种灵芝提取物体内外抗肿瘤作用。方法应用小鼠H22肝癌移植瘤模型,研究灵芝提取物的体内抗肿瘤作用;采用MTT法检测灵芝提取物的体外抗肿瘤活性。结果①灵芝提取物对H22移植瘤呈剂量依赖性抑制作用,500 mg.kg-1组的瘤重抑制百分率达59.3%,与阴性对照组比较有统计学差异（P〈0.01）;②MTT法检测显示灵芝提取物对K562和HL60两种瘤株有较强抑制作用,对SMMC7221、HepG2、SW480、SW1116和SGC7901五种瘤株抑制作用较弱。结论灵芝提取物在体外及体内均有较强的抗肿瘤作用,其有效成分及机制有待进一步研究。

Experimental Study on Anti-tumor Effects of Extracts from Ganoderma Lucidum

OBJECTIVE To explore anti-tumor effects of extracts from Ganoderma Lucidum(GLE).METHODS Anticancer effects of GLE were experimented by mice bearing H22 hepatocarcinoma transplantation tumor model in vivo and MTT reduction test in vitro.RESULTS ①GLE could inhibit H22 transplanted tumor growth in mice in a dose-dependent manner,the inhibitive percentage of carcinoma weight was 59.3% in 500 mg·kg-1 group and there was obviously differentiation when comparing with negative control group(P<0.01).②GLE had an inhibitory effect on the proliferation of K562 and HL60 human tumor cells and had less inhibitory activity on SMMC7221,HepG2,SW480,SW1116 and SGC7901 human tumor cells.CONCLUSION This study confirms that GLE has significant anticancer effect in vitro and vivo.However,the mechanism and active ingredients remain to be studied.