| 荧光光谱法研究Ca2+存在下左氧氟沙星与人血清白蛋白的结合作用 |
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| 引用本文： | 王志强,胡卢丰,吴继禹,王学宝,黄学荪,张秀华.荧光光谱法研究Ca2+存在下左氧氟沙星与人血清白蛋白的结合作用[J].中国药师,2011,14(7):922-925. |
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| 作者姓名： | 王志强 胡卢丰 吴继禹 王学宝 黄学荪 张秀华 |
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| 作者单位： | 1. 温州医学院附属第一医院药剂科,浙江温州,325000
2. 温州医学院药学院分析测试中心 |
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| 摘 要： | 目的：研究左氧氟沙星对人血清白蛋白以及在ca^2＋存在下左氧氟沙星与人血清白蛋白的结合作用。方法：通过荧光光谱法分析了左氧氟沙星对人血清白蛋白以及ca^2＋存在条件下左氧氟沙星对人血清白蛋白荧光淬灭光谱、同步荧光光谱，根据热力学方程讨论两者间主要的作用力类型。结果：在生理条件（pH=7．4，37℃）下，根据Stem—Volmer方程和荧光淬灭双倒数图，确定了左氧氟沙星对人血清白蛋白淬灭类型为静态淬灭，左氧氟沙星对人血清白蛋白的结合常数K=1．46×10^5L·mol^-1，结合位点n=1．1，根据热力学方法确定作用力类型为疏水作用力；在ca^2＋存在条件下，淬灭类型和作用力类型不变，结合常数K=2．38×10^4L·mol^-1，结合位点n：1．02。结论：在ca^2＋存在条件下，左氧氟沙星对人血清白蛋白的荧光淬灭减弱，结合常数和结合位点均变小。为研究左氧氟沙星的生物学效应，以及左氧氟沙星和Ca^2＋对蛋白质构象的影响等提供了重要信息。
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| 关 键 词： | 左氧氟沙星 人血白蛋白 荧光猝灭 |
| 收稿时间： | 1/5/2011 12:00:00 AM |
| 修稿时间： | 4/9/2011 12:00:00 AM |
Study on the Binding Interaction of Levofloxacin with Human Serum Albumin in the Presence of Ca2+ by Fluorescence Spectrophotometry |
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| Wang Zhiqiang,Hu Lufeng,Wu Jiyu,Wang Xue-bao,Huang Xuesun and Zhang Xiuhua.Study on the Binding Interaction of Levofloxacin with Human Serum Albumin in the Presence of Ca2+ by Fluorescence Spectrophotometry[J].China Pharmacist,2011,14(7):922-925. |
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| Authors: | Wang Zhiqiang Hu Lufeng Wu Jiyu Wang Xue-bao Huang Xuesun and Zhang Xiuhua |
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| Institution: | Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical College, Zhejiang Wenzhou 325000, China;Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical College, Zhejiang Wenzhou 325000, China;Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical College, Zhejiang Wenzhou 325000, China;Analytical and Testing Center of Wenzhou Medical College;Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical College, Zhejiang Wenzhou 325000, China;Department of Pharmacy, The First Affiliated Hospital of Wenzhou Medical College, Zhejiang Wenzhou 325000, China |
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| Abstract: | Objective:To investigate the binding interaction of levofloxacin with human serum albumin in the presence of Ca~(2+)by fluorescence spectrophotometry.Method:The interaction of levofloxacin with human serum albumin(HSA)in the presence of Ca~(2+) was studied by measuring fluorescence quenching spectra and synchronous fluorescence spectra.The type of binding force was estimated by the calculation of thermodynamic parameters.Result:After analyzing the fluorescence quenching data according to Stem-Volmer equatio... |
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| Keywords: | Levofloxacin human serum albumin fluorescence quenching |
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