CO2对宫颈癌HeLa细胞抗失巢凋亡特性的影响

 目的 观察CO₂对宫颈癌HeLa细胞系抗失巢凋亡能力的影响。方法 以宫颈癌HeLa细胞系为研究对象，实验组给予CO₂持续通气4小时，压力为8mmHg,对照组HeLa细胞不进行CO₂通气，两组细胞同时设贴壁培养组和悬浮培养组进行培养24h、72h、120h。利用软琼脂集落形成实验、Poly HEMA悬浮培养、流式细胞技术、观察体外培养过程中CO₂对宫颈癌HeLa细胞系增殖和抗失巢凋亡的影响。结果 实验组与对照组均具有凋亡能力和抗失巢凋亡能力，实验组的增殖能力和集落形成明显优于对照组 （P＜0.01 )，但实验组抗失巢凋亡能力明显低于对照组。结论 CO₂能够促进宫颈癌HeLa细胞集落形成，具有较强的失巢凋亡能力，但不能增强抗失巢凋亡能力。

Effect of CO2 on Anti-anoikis of HeLa Cell

Objective To observe the effect of CO₂ on the anti-anoikis of HeLa cell (cervical cancer). Methods HeLa cells were randomly divided into experimental group and control group. In experimental group, CO₂ was insufflated into the containers by pressures of 8 mm Hg for 4 hours. Control group was not treated by CO₂. Two groups of cells were cultured for 24h, 72h and 120h in adherent and suspension methods. The effect of CO₂ on the proliferation and antianoikis of HeLa cells were detected through soft agar colony formation experiments, Poly HEMA suspension culture and Flow cytometry assay.ResultsThe abilities of apoptosis and anti-anoikis were observed in 2 groups. The proliferation and colony formation capabilities were stronger in experimental group than that in control group, but the anti anoikis of experiment group was significantly lower than that of the control group(P＜0.01).Conclusion CO₂ was able to improve the colony formation and anoikis of HeLa cells, no a great impact, however, on anti-anoikis.