金黄色葡萄球菌临床分离株肠毒素基因的检测

摘要]目的 检测临床分离的金黄色葡萄球菌肠毒素基因，了解肠毒素基因的携带情况。方法 根据Genbank的序列设计合成金黄色葡萄球菌肠毒素基因A-F的引物，采用聚合酶链反应方法对随机收集的124株金黄色葡萄球菌肠毒素基因进行检测，并进行序列分析。结果 在124株受检的金黄色葡萄球菌中，116株检出肠毒素基因，检出率为93.5%，其中SEA、SEB、SEC、SED和SEF的检出率分别为90.5%、6.9%、61.3%、5.2%和25.9%，未检测出SEE基因。同时检出2种及2种以上的肠毒素基因的菌株有78株（67.2%），以携带SEA+SEC、SEA+SEF和SEA+SEC+SEF基因为主，检出率分别为33.6%，7.8%，13.8%。序列分析显示所检出的肠毒素基因与Genbank上登陆的参考序列的同源性为97%～100%。结论 金黄色葡萄球菌临床分离株肠毒素基因携带率高，其中以携带SEA、SEC、SEF基因为主。

Study on detection of Staphylococcus aureus enterotoxin gene isolated from clinic

Abstract] Objective To detect the exterotoxin genes of Staphylococcus aureus from clinical specimens and analyze the carrier condition of exterotoxin genes. Method The primers of exterotoxin genes A～F of Staphylococcus aureus were designed and synthesized according to the enterotoxin sequences in the Genbank. And then Theses enterotoxin genes of 124 Staphylococcus aureus that isolated from clinical sources were identified by polymerase chain reaction (PCR) and the gene was sequenced and analyzed. Result 116 of 124 strains Staphylococcus aureus had enterotoxin gene, the detection rate was 93.5%, and the detection rate of SEA, SEB, SEC, SED and SEF was 90.5%,6.9%,61.3%,5.2%,25.9% and 93.5% with none of SEE gene positives. In these strains, there were 78 strains with carrying 2 and 2 or more kinds of enterotoxines, and the positive rate was 67.2%. Furthermore, the main genotypes were SEA and SEC genes, SEA and SEF genes, SEA and SEC and SEF genes, the detection rates were 33.6%, 7.8% and 13.8%, respectively. Compared with sequences of enterotoxin of Staphylococcus aureus registered in Genbank, there was from 97% to 100% identities. Conclusion The proportion of entoertoxin genes positive Staphylococcus aureus strains is very high in clinical, in which the main genotypes are SEA, SEC and SEF.