辛伐他汀诱导促进骨形成蛋白-7基因修饰的兔间充质干细胞的成骨转化

目的:研究辛伐他汀诱导对骨形成蛋白-7(BMP-7)基因修饰的间充质干细胞(MSCs)增殖和分化表型的影响。方法:将辛伐他汀诱导和未经诱导的兔MSCs分别转导BMP-7基因后,Westernblotting检测转导后细胞BMP-7的表达;通过观察细胞生长以及碱性磷酸酶活性和测定骨钙素含量,分析辛伐他汀诱导对BMP-7基因修饰的MSCs的增殖和分化表型的影响。结果:转导后BMP-7基因在诱导组和未诱导组MSCs中均有表达,诱导组细胞BMP-7表达量、碱性磷酸酶活性和骨钙素含量均比较未诱导组明显增高。结论:转导前辛伐他汀诱导能够促进BMP-7基因修饰的MSCs的成骨转化。

Osteoblastic differentiation induced by simvastatin promote bone morphogenic protein-7 modified mesenchymal stem cells osteoblast conversion

Objective To determine whether or not the induction of simvastatin influences proliferation and differentiation of the phenotype of bone morphogenic protein-7(BMP-7) modified rabbit mesenchymal stem cells(rMSCs).Methods The rMSCs treated and untreated with simvastatin were transfected with BMP-7 gene.The expression of BMP-7 was determined by Western blotting.The changes of proliferation and differentiation of the phenotype in the cells were observed by cell growth state, alkaline phosphatase(ALP) activity a...