| 西罗莫司对膀胱癌BIU-87细胞增殖及侵袭能力的影响 |
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| 引用本文： | 单广夷,;付成,;陈昂,;胡滨,;毕缓,;陈启光. 西罗莫司对膀胱癌BIU-87细胞增殖及侵袭能力的影响[J]. 解剖科学进展, 2014, 0(5): 440-442 |
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| 作者姓名： | 单广夷, 付成, 陈昂, 胡滨, 毕缓, 陈启光 |
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| 作者单位： | [1]辽宁省肿瘤医院泌尿外科,辽宁沈阳110024; [2]中国医科大学附属第一医院泌尿外科,辽宁沈阳110001 |
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| 摘 要： | 目的探讨西罗莫司对膀胱癌BIU-87细胞增殖和侵袭能力的作用。方法将BIU-87细胞贴壁培养于10%胎牛血清、100 U/ml青霉素、100μg/ml链霉素的RPMI-1640培养液中,37℃、5%CO饱和湿度培养箱中2培养。取对数生长的BIU-87细胞,处理组加入西罗莫司的浓度范围是(10-1000)nmol/L;对照组不加药物,每天紧更换1640培养液。MTT法分析各浓度组的生长抑制率并制作抑制曲线,Transwell法通过使用matrigel基质胶检测BIU-87细胞在体外的侵袭能力。结果西罗莫司对BIU-87细胞有明显抑制作用,呈时间和量效依赖关系。Transwell法发现不同浓度的西罗莫司可以减弱BIU-87细胞的体外侵袭能力,呈量效依赖性。结论西罗莫司可以抑制膀胱癌BIU-87细胞的增殖活性及侵袭能力。
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| 关 键 词： | 西罗莫司 BIU-87细胞 增殖 侵袭 |
Sirolimus inhibits proliferation and invasion of human bladder carcinoma cell line BIU-87 |
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| Affiliation: | SHAN Guang-yi, FU Cheng , HEN Ang, HU Bin, BI Huan, CHEN Qi-guang ( 1. Department of Urology, Liaoning Cancer Hospital ＆ Institute, Shenyang 110024, China; 2. Department of Urology, the First Hospital of Chirm Medical University, Shenyang 110001, China ) |
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| Abstract: | Objective To study the effects of Sirolimus on proliferation and invasion of human bladder cancer BIU-87 cells. Methods BIU-87 cells were cultured in RPMI-1640 containing 10% fetal bovine serum and penicillin/streptomycin at 37℃ in a 5% CO2 incubator. BIU-87 cells of logarithmic growth phase were used and treated with various concentrations of Sirolimus solntion（10-1000nmol/L）, cells in control group were only treated with RPMI-1640. The growth inhibition rate of BIU-87 cells was measured by MTT method, the invasion rate of BIU-87 cells was measured by Transwell chamber assay. Results Sirolimus inhibited the proliferation of BIU-87 cells in time and dose-dependent manners. Sirolimus inhibited the of BIU-87 cells by Transwell chamber assay. Conclusion Sirolimus inhibited the growth and invasiveness of BIU-87 bladder carcinoma cells. |
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| Keywords: | sirolimus BIU-87 cell proliferation invasion |
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